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Mass spectrometry-based draft of the soybean proteome

ABSTRACT: Soybean is a critical source of vegetable protein, yet its proteome remains under-characterized. Here, we quantify 12,855 proteins across 14 soybean organs using 4D-data-independent acquisition mass spectrometry (4D-DIA-MS), creating the most extensive soybean proteome dataset to date. Organ-specific protein expression and co-expression analyses highlight functional specificity, with significant differences in protein-transcript abundance across organs. We also map N6-methyladenosine (m6A) modifications, identifying their key role in post-transcriptional protein regulation. Integrative analysis of proteome and m6A methylome identifies novel regulator in m6A methylation. This comprehensive proteomic and m6A landscape advances our understanding of soybean biology and provides a valuable resource for crop improvement.

ORGANISM(S): Glycine Max

SUBMITTER: Zhe Liang

PROVIDER: PXD048672 | iProX | Thu Jan 18 00:00:00 GMT 2024

REPOSITORIES: iProX

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