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Proteome of hrsc1 seed at four different developmental stages

ABSTRACT: The grains of mutant hrsc1 exhibited a severe chalkiness phenotype relative to its wild type QZ-10. Physiological measurements and scanning electron microscopy observation further indicated that hrsc1 grains contain oval irregular starch granules, as well as significantly increased RS (up to 14-fold), amylose (up to 1.6-fold), lipid (up to 3-fold) and protein (up to 1.2-fold) contents. Dynamic proteome of grains were analyzed on four different development stages (DAF 6d, 12d, 18d and 24d) during filling stage between hrsc1 and QZ-10.

ORGANISM(S): Oryza Sativa

SUBMITTER: Rongyu Huang

PROVIDER: PXD049027 | iProX | Wed Jan 31 00:00:00 GMT 2024

REPOSITORIES: iProX

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Project description:Heat stress occurrence during endosperm development and seed filling forms chalky portion in the limited zone of starchy endosperm of rice grains. In this study, isolation of aleurone, dorsal, central and lateral tissues of developing endosperm by laser-microdissection (LM) coupled with gene expression analysis of 44K microarray was performed to identify key regulatory genes involved in the formation of milky-white (MW) and white-back (WB) chalky grains during heat stress. Gene regulatory network analysis classified the genes changed under heat stress into five modules. the modules of genes changed in developing starchy endosperm corresponding to MW and WB portion under heat stress suggested different regulatory genes involved in each type of grain chalk. The most distinct expression pattern was observed in a M1 and M2 modules where most of the small heat shock proteins and cellular organisation genes being upregulated under heat stress in dorsal aleurone cells and dorsal starchy endosperm zones The histological observation supported the significant increase in cell number and size of dorsal aleurone cells in WB grains. With regard to the central zone of starchy endosperm, preferential downregulation of high molecular weight heat shock proteins (HMW HSPs) including a prominent member encoding for endoplasmic reticulum (ER) chaperones by heat stress were observed, while expression of starch-biosynthesis genes remained unaffected. Characterization of transgenic plants supressing endosperm lumenal binding protein (BiP1), an ER chaperone preferentially downregulated at MW portion under heat stress, showed an evidence of forming the chalky grains without disturbing the expression of starch-biosynthesis genes. The present LM-based comprehensive expression analyses provides novel inferences that HMW HSPs play important role in controlling the redox, nitrogen and amino aicd metabolism in endosperm leading to the formation of MW and WB chalky grains.Keywords ; developing endosperm, gene expression, grain chalkiness, heat stress, laser-microdissection, Oryza sativa.

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Proteome of hrsc1 seed at four different developmental stages

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