Dataset Information

Proteomic analysis of PDCoV-infected HIEC-6 cells

ABSTRACT: Data-independent acquisition (DIA) is a new type of mass spectrometry data acquisition developed in recent years. The entire scanning range of mass spectrometry can be divided into several windows based on the mass-to-charge ratio (m/z), and then all the parent ions in each window are fragmented and detected, and the fragment ion information of all the parent ions is collected for protein characterization and quantification. Currently popular proteomics research tools, such as iTRAQ/TMT, Label-free, SILAC use a data-dependent acquisition (DDA) mode of scanning, the first level of mass spectrometry for full scanning acquisition of parent ion signals to generate the first level of spectrum, the second level of mass spectrometry to select the first level of the signal intensity in the spectrum in turn In the secondary mass spectrometry, the peaks ranked as top10/20/40 in the primary spectrum were fragmented and the corresponding daughter ions were collected.

ORGANISM(S): Homo Sapiens

SUBMITTER: Yuhang Jiang

PROVIDER: PXD049713 | iProX | Tue Feb 20 00:00:00 GMT 2024

REPOSITORIES: iProX

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Publications

A novel host restriction factor MRPS6 mediates the inhibition of PDCoV infection in HIEC-6 cells.

Jiang Yuhang Y Zhang Guoqing G Li Letian L Chen Jing J Hao Pengfei P Gao Zihan Z Hao Jiayi J Xu Zhiqiang Z Wang Maopeng M Li Chang C Jin Ningyi N

Frontiers in immunology 20240611

<h4>Introduction</h4>Porcine deltacoronavirus (PDCoV) is a zoonotic pathogen with a global distribution, capable of infecting both pigs and humans. To mitigate the risk of cross-species transmission and potential outbreaks, it is crucial to characterize novel antiviral genes, particularly those from human hosts.<h4>Methods</h4>This research used HIEC-6 to investigate PDCoV infection. HIEC-6 cells were infected with PDCoV. Samples were collected 48 h postinfection for proteomic analysis.<h4>Resul ...[more]

PMID: 38919620

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Dataset Information

Proteomic analysis of PDCoV-infected HIEC-6 cells

Publications

A novel host restriction factor MRPS6 mediates the inhibition of PDCoV infection in HIEC-6 cells.

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