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Multiple LC-MS platforms analysis under standardized quality control system of urinary proteomics for precision medicine studies

ABSTRACT: In this study, we carried out a comprehensive evaluation of multiple LC-MS platforms in urinary proteomics including 720 proteome measurements. A series of interrelated studies was designed to assess the intra- and inter-platform consistency and reproducibility. Firstly, a uniformly prepared urine sample is distributed to 20 LC-MS platforms, where a total of 160 data are generated to analyze the consistency and reproducibility across different LC-MS platforms. Secondly, benchmarking samples, which consist of tryptic digests of human, yeast, and E. coli proteins mixed in defined proportions, are generated to mimic differential expressed biological samples and provide proof of the robustness and reproducibility of the different LC-MS platforms. Finally, the above methodologies are applied to actual large cohort clinical CRC urinary proteome datasets derived from 3 LC-MS platforms to further demonstrate the performance of urinary proteomics cohorts from multi-platform in biomarker discovery.

ORGANISM(S): Homo Sapiens Escherichia Coli Saccharomyces Cerevisiae

SUBMITTER: Wei Sun

PROVIDER: PXD050291 | iProX | Fri Mar 01 00:00:00 GMT 2024

REPOSITORIES: iProX

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