Dataset Information

Tandem Mass Tag-based proteomics of astaxanthin-producing tobacco expressing astaxanthin synthetases from Adonis aestivalis

ABSTRACT: The synthesis of astaxanthin leads to growth retardation in tobacco. Meanwhile, irregular mosaic pigmentation randomly appears on the leaves of astaxanthin-producing tobacco. The purpose of this project is to elucidate the mechanism of growth retardation in astaxanthin-producing tobacco and to compare the differences between mosaic pigmentation regions on astaxnathin-producing tobacco leaves.

ORGANISM(S): Nicotiana Tabacum

SUBMITTER: Hongbo Zhang

PROVIDER: PXD050349 | iProX | Tue Mar 05 00:00:00 GMT 2024

REPOSITORIES: iProX

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Publications

Multi-Omics Analysis Provides Insights into a Mosaic-Leaf Phenotype of Astaxanthin-Producing Tobacco.

Wang Jialin J Du Zaifeng Z Lin Xiaoyang X Li Peng P Sun Shihao S Yang Changqing C Chen Yong Y Zhang Zhongfeng Z Yin Xue X Fang Ning N

Plants (Basel, Switzerland) 20250319 6

In metabolically engineered plants, the target products are usually uniformly distributed in the whole plant or specific tissues. When engineering tobacco to produce astaxanthin, a ketocarotenoid with strong antioxidant activity and multiple bioactivities, a scattered distribution of astaxanthin-producing regions was observed in a small portion of astaxanthin-producing tobacco plants, which caused mosaic-like red and green spots on the leaves (ASTA-mosaic). A physiological assay showed that the ...[more]

PMID: 40265925

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Project description:Comparative analysis of tobacco leaves transcriptomes unveils carotenoid pathway potentially determined the characteristics of aroma compounds in different environmental regions. Tobacco (Nicotiana tabacum) is a sensitive crop to environmental changes, and a tobacco with unique volatile aroma fractions always formed in specific ecological conditions. In order to investigate the differential expressed genes caused by environmental changes and reveal the formation mechanism of characteristics of tobacco in three different aroma tobacco regions of Guizhou Province, Agilent tobacco microarray was adapted for transcriptome comparison of tobacco leaves in medium aroma tobacco region Kaiyang and light aroma tobacco regions Weining and Tianzhu. Results showed that there was big difference among the gene expression profiles of tobacco leaves in different environmental conditions. A total of 517 differential expressed genes (DEGs) between Weining and Tianzhu were identified, while 733 and 1,005 genes differentially expressed between Longgang and another two tobacco regions Weining and Tianzhu, respectively. Compared with Longgang, up-regulated genes in Weining and Tianzhu were likely involved in secondary metabolism pathways, especially carotenoid pathway, including PHYTOENE SYNTHASE, PHYTOENE DEHYDROGENASE, LYCOPENE ε-CYCLASE, CAROTENOID β-HYDROXYLASE and CAROTENOID CLEAVAGE DIOXYGENASE 1 genes, while most down-regulated genes played important roles in response to temperature and light radiation, such as heat shock proteins. Gene Ontology and MapMan analyses demonstrated that the DEGs among different environmental regions were significantly enriched in light reaction of photosystem II, response of stimulus and secondary metabolism, suggesting they played crucial roles in environmental adaptation and accumulation of aroma compounds in tobacco plants. Through comprehensive transcriptome comparison, we not only identified several stress response genes in tobacco leaves from different environmental regions but also highlighted the importance of carotenoid pathway genes for characteristics of aroma compounds in specific growing regions. Our study primarily laid the foundation for further understanding the molecular mechanism of environmental adaptation of tobacco plants and molecular regulation of aroma substances in tobacco leaves.

Project description:Carotenoids are essential pigments in photosynthesis and other physiological processes, present in various organisms. Microalgae are considered an important natural source for the commercial production of some of these compounds. The accumulation of the carotenoids astaxanthin and lutein in response to nitrogen and light availability has been optimized in continuous photoautotrophic cultures of Chromochloris zofingiensis. Astaxanthin cellular content increased significantly as the nitrate concentration in the feed medium decreased, while that of lutein remained largely unchanged. The cellular levels of lutein and astaxanthin were inversely correlated with the nitrogen content of the biomass. Thus, for 4% N in the dry biomass, accumulation of astaxanthin was highest and that of lutein lowest. The opposite was recorded for 9% N. Average irradiance positively affected lutein accumulation at both high and low nitrate, whereas that of astaxanthin did only increase under nitrogen-limiting conditions. An integrated analysis of transcriptomics and carotenoid profiling data provides evidence that the response to nitrogen is more significant than that to light availability, and that a synergy exists between both factors, with enhancement of their individual effects. Regulation appears to be orchestrated at several levels: the expression pattern of cyclases suggests a redirection of lycopene to β-carotene, which is converted into astaxanthin through overexpression of BKT1. In turn, slightly higher lutein values are achieved by altering the α-carotene/lutein ratio, due to upregulation of CYP97A1 and CYP97C, especially under high irradiance conditions. The differential responses of astaxanthin and lutein to nitrogen and light availability could enable the programmed generation of C. zofingiensis biomass in continuous culture with a specific astaxanthin to lutein ratio, through careful adjustment of the nitrate concentration in the feed medium, using light irradiance as a modulator.

Dataset Information

Tandem Mass Tag-based proteomics of astaxanthin-producing tobacco expressing astaxanthin synthetases from Adonis aestivalis

Publications

Multi-Omics Analysis Provides Insights into a Mosaic-Leaf Phenotype of Astaxanthin-Producing Tobacco.

Similar Datasets

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