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Proteomic of control and FBL knockdown C4-2 cells

ABSTRACT: We employed a quantitative proteomics approach to compare the protein expression levels between control and FBL knockdown C4-2 cells, revealing expression levels of some proteins which have decreased mRNA half-life.

ORGANISM(S): Homo Sapiens

SUBMITTER: Kaifu Chen

PROVIDER: PXD050701 | iProX | Sun Mar 17 00:00:00 GMT 2024

REPOSITORIES: iProX

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2'-O-methylation at internal sites on mRNA promotes mRNA stability.

Li Yanqiang Y Yi Yang Y Gao Xinlei X Wang Xin X Zhao Dongyu D Wang Rui R Zhang Li-Sheng LS Gao Boyang B Zhang Yadong Y Zhang Lili L Cao Qi Q Chen Kaifu K

Molecular cell 20240601 12

2'-O-methylation (Nm) is a prominent RNA modification well known in noncoding RNAs and more recently also found at many mRNA internal sites. However, their function and base-resolution stoichiometry remain underexplored. Here, we investigate the transcriptome-wide effect of internal site Nm on mRNA stability. Combining nanopore sequencing with our developed machine learning method, NanoNm, we identify thousands of Nm sites on mRNAs with a single-base resolution. We observe a positive effect of F ...[more]

PMID: 38906115

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