Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Intact Mass analysis of class III lanthipeptide PneA

ABSTRACT: Intact Mass analysis of PneA under various modification states

ORGANISM(S): Streptococcus Pneumoniae Pt8114

SUBMITTER: Min Luo

PROVIDER: PXD050940 | iProX | Tue Aug 13 00:00:00 BST 2024

REPOSITORIES: iProX

ACCESS DATA

Json Xml

Publications

Mechanistic insights into lanthipeptide modification by a distinct subclass of LanKC enzyme that forms dimers.

Li Yifan Y Shao Kai K Li Zhaoxing Z Zhu Kongfu K Gan Bee Koon BK Shi Jian J Xiao Yibei Y Luo Min M

Nature communications 20240817 1

Naturally occurring lanthipeptides, peptides post-translationally modified by various enzymes, hold significant promise as antibiotics. Despite extensive biochemical and structural studies, the events preceding peptide modification remain poorly understood. Here, we identify a distinct subclass of lanthionine synthetase KC (LanKC) enzymes with distinct structural and functional characteristics. We show that PneKC, a member of this subclass, forms a dimer and possesses GTPase activity. Through th ...[more]

PMID: 39154050

Similar Datasets

Glycosylation Profiling of Neoplastic Biomarker Alpha Fetoprotein Through Intact Mass Protein Analysis

Project description:Glycosylation is an important post-translational modification (PTM) mechanism of proteins, that modulates protein function. Measurement of clinically adopted cancer protein biomarkers is commonly performed using affinity-based techniques, which often suffer from poor specificity and inability to distinguish between closely related proteoforms. Alpha fetoprotein (AFP) is a circulating cancer biomarker implicated in multiple neoplastic malignancies, including hepatocellular carcinoma (HCC). AFP glycoforms with core fucosylation structure (AFP-L3) are used clinically as a biomarker, to predict risk of developing HCC and to monitor its recurrence. Mature AFP has a single glycosylation site, while there is a high degree of structural variation in AFP-glycan modifications. Because AFP has single glycosylation site, masses of intact AFP proteoforms can be used to differentiate and identify PTM in their native states. We developed a method for intact AFP glycoform analysis that utilizes AFP-specific immune-enrichment, followed by LC-high resolution mass spectrometry (LC-HRMS) analysis to differentiate and quantitate the relative abundance of AFP glycoforms and evaluated the method’s performance.

2023-01-11 | PXD038602 | Pride

Glycosylation Profiling of Neoplastic Biomarker Alpha Fetoprotein Through Intact Mass Protein Analysis

Project description:Alpha fetoprotein (AFP) is a circulating cancer biomarker implicated in multiple neoplastic malignancies, including hepatocellular carcinoma (HCC). AFP glycoforms with core fucosylation structure (AFP-L3) are used clinically as a biomarker, to predict risk of developing HCC and to monitor its recurrence. Mature AFP has a single glycosylation site, while there is a high degree of structural variation in AFP-glycan modifications. Because AFP has single glycosylation site, masses of intact AFP proteoforms can be used to differentiate and identify PTM in their native states. We developed a method for intact AFP glycoform analysis that utilizes AFP-specific immune-enrichment, followed by LC-high resolution mass spectrometry (LC-HRMS) analysis to differentiate and quantitate the relative abundance of AFP glycoforms and evaluated the method’s performance.

2023-01-11 | PXD038606 | Pride

Intact mass and middle-down analysis for mAbs

Project description:An integrated strategy has been developed to characterize Trastuzumab and Adalimumab structural heterogeneity, which has prominent advantages in fast sample preparation with minimal artifacts, and complementary information obtained from intact mass and middle-down analyses.

2020-09-03 | PXD021287 |

Modification of fat mass through targeting distal enhancer of Ptrf

Project description:Modification of fat mass through targeting distal enhancer of Ptrf

| PRJNA1106358 | ENA

Role of microRNAs in compensatory b-cell mass expansion associated with pregnancy and obesity

Project description:We found that in rodents, b-cell mass expansion during pregnancy and obesity is associated with changes in the expression of a group of islet microRNAs. We were able to reproduce in isolated pancreatic islets the decrease of miR-338-3p level observed in gestation and obesity by activating the G-protein coupled estrogen receptor GPR30 and the GLP1 receptor. Blockade of miR-338-3p in b-cells using specific anti-miR molecules mimicked gene expression changes occurring during b-cell mass expansion and resulted in increased proliferation and improved survival both in vitro and in vivo. These findings point to a major role for miR-338-3p in compensatory b-cell mass expansion occurring under different insulin resistance states.

2012-11-20 | GSE36067 | GEO

IntAct- IFA to prevent anastomotic leak in rectal cancer surgery

Project description:See https://www.cancerresearchuk.org/about-cancer/find-a-clinical-trial/a-study-looking-to-reduce-a-serious-side-effect-of-rectal-cancer-surgery-intact

| 2410037 | ecrin-mdr-crc

Global quantification of intact proteins via chemical isotope labeling and mass spectrometry

Project description:Although thousands of intact proteins can be feasibly identified in recent years, global quantification of intact proteins is still challenging. Herein, we develop a high-throughput strategy for global intact protein quantification based on chemical isotope labeling. The isotope incorporation efficiency is as high as 99.2% for complex intact protein samples extracted from HeLa cells. Further, the pTop 2.0 software is developed for automated quantification of intact proteoforms in a high-throughput manner. The high quantification accuracy and reproducibility of this strategy have been demonstrated for both standard and complex cellular protein samples. A total of 2,283 intact proteoforms originated from 660 protein accessions are successfully quantified under anaerobic and aerobic conditions and the differentially expressed proteins are observed to be involved into the important biological processes such as stress response.

2019-04-30 | PXD012526 | Pride

Non-intact membrane DNBR

Project description:Non-intact membrane DNBR Raw sequence reads

| PRJNA954296 | ENA

Intact mass LC-MS analysis of Flu B HA1 and HA2 proteins

Project description:To determine the sequences of the Flu HA1 and HA2 proteins, we performed intact mass LC-MS analysis. By reducing disulfide bridges two peaks corresponding to the HA1 and HA2 components were detected. Mass profiles were obtained for both glycosylated and deglycosylated HA proteins.

2025-05-06 | PXD046130 | Pride

Mass culture of rotifer

Project description:Mass culture of rotifer Metagenome

| PRJNA1123473 | ENA