Mining novel gene targets for improving tolerance to furfural and acetic acid in Yarrowia lipolytica using whole-genome clustered regularly interspaced short palindromic repeats interference library

ABSTRACT: In this study, a whole-genome CRISPRi library was developed in Y. lipolytica and applied for enforcing the tolerance to furfural and acetic acid. Several novel gene targets were discovered through NGS before and after screening. And to analyze the tolerance mechanism caused by transcriptional repression of the most prominent gene targets, the transcriptome and proteomics were performed.

ORGANISM(S): Yarrowia Lipolytica

SUBMITTER: Yingxiu Cao  

PROVIDER: PXD051116 | iProX | Sun Mar 31 00:00:00 GMT 2024

REPOSITORIES: iProX