Dataset Information

Proteomics profiling of the honeybee parasite Tropilaelpas mercedesae across post-embryonic development

ABSTRACT: Using ultra-high resolution mass spectrometry, we provide a comprehensive proteomic profile of T. mercedesae across all post-embryonic developmental stages (i.e., protonymphs, deutonymphs, adults, and reproductive mites).

ORGANISM(S): Tropilaelaps Mercedesae

SUBMITTER: Shufa Xu

PROVIDER: PXD051356 | iProX | Thu Apr 11 00:00:00 BST 2024

REPOSITORIES: iProX

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Publications

Proteomics profiling of the honeybee parasite Tropilaelaps mercedesae across post-embryonic development.

Wei Qiaohong Q Wu Jiangli J Liu Fengying F Sun Jiajing J Kang Weipeng W Zhao Meijiao M Wang Feng F Zhang Chenhuan C Xu Shufa S Han Bin B

Scientific data 20240515 1

Tropilaelaps mercedesae, an ectoparasitic mite of honeybees, is currently a severe health risk to Apis mellifera colonies in Asia and a potential threat to the global apiculture industry. However, our understanding of the physiological and developmental regulation of this pest remains significantly insufficient. Using ultra-high resolution mass spectrometry, we provide the first comprehensive proteomic profile of T. mercedesae spanning its entire post-embryonic ontogeny, including protonymphs, d ...[more]

PMID: 38750068

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Project description:The TET dioxygenases erase mediate DNA dedemethylation in pre-implantation embryos and in primordial germ cells, yet limited studies address their contribution to the global gain of DNA methylation following implantation. Here, we show that Tet1 is expressed and non-redundantly contributes to 5-hydroxymethylctyosine (5hmC) non-redundantly in the pre-gastrulation mouse epiblast. Ablation of Tet1 in primed epiblast cells results in widespread loss of 5hmC associated with gain of 5-methylcytosine at CpG islands and promoters. Moreover, Tet1 is expressed, albeit at lower levels, in the extra-embryonic ectoderm. Tet1-deficiency in the pre-streak mouse embryos causes dysregulation of early lineage regulators in the epiblast and increased expression of metabolic genes in the extra-embryonic ectoderm. Our studies reveal a distinct role of Tet1 in regulating the methylome landscape of the post-implantation mammalian epiblast and a hitherto unknown gene repressive effect in the extra-embryonic lineage, providing insights into the early developmental origins of epigenetic-based basis of imprinting and developmental disorders. Lysates of E6.25 epiblast (E) and extra-embryonic (ExE) tissues were pooled, based on retrospective genotyping, from at least 6 embryos (collected from 2-3 litters). Four pooled biological replicates were collected for each tissue compartment and each genotype, i.e. wild type (wt), Tet1gt/wt (Zwt), Tet1gt/gt(ZZ); in 3 of 4 pooled replicates, the E and ExE samples were obtained from the same litters and given the same numbering. One ExE sample of ZZ was lost. RNA was purified using the RNeasy micro Kit (Qiagen) to obtain 1-10 ng of RNA per pool. RNA quality was assessed using a Bioanalyzer 6000 Pico chip and only samples with RNA integrity number (RIN) > 8.0 were processed further. First-strand cDNA synthesis and amplification was performed by using the SMARTer Ultra Low InputÂ RNA amplification kit v3 (Clontech). Libraries were prepared using NEBNext Ultra DNA library prep for Illumina and sequenced on NextSeq 500 in high-output to generate 75-bp single-end reads.Â Â Low quality ends and adapter sequences wereÂ trimmed off from the Illumina reads withÂ FastX 0.0.13 andÂ cutadaptÂ 1.7.1. Using FastX andÂ ShortRead 1.16.3, we filtered subsequently small readsÂ (length < 35 bp), polyA-reads (>90% of the bases equal A),Â ambiguous reads (containing N) and lowÂ quality reads (>50% of theÂ bases < Q25).

Dataset Information

Proteomics profiling of the honeybee parasite Tropilaelpas mercedesae across post-embryonic development

Publications

Proteomics profiling of the honeybee parasite Tropilaelaps mercedesae across post-embryonic development.

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