Dataset Information

Characterization of the peptidome in the spermosphere of common bean (Phaseolus vulgaris)

ABSTRACT: Seeds are crucial for plant reproduction, dispersal, and agriculture. Seed quality and vigour greatly impact crop production, referring to their ability to germinate rapidly and uniformly under varying environmental conditions, producing healthy seedlings that can withstand biotic and abiotic stress accentuated by global climate change. During germination, seeds release exudates, complex mixtures of organic and inorganic molecules, into the micro-environment surrounding them, known as the spermosphere. These exudates play a pivotal role in seedling development and overall plant fitness by influencing microbial selection, growth, and interactions in the spermosphere, ultimately shaping the plant's microbiome. Our research demonstrated that germinating seeds release diverse metabolites, peptides, proteins, and small RNAs in the exudates, some with functional properties like antimicrobial or antioxidant activities. To understand the composition and functional properties of germinating seed exudates, we conducted our study using eight common bean seed genotypes produced under two contrasted locations in France. We investigated the diversity of peptides in the spermosphere.

ORGANISM(S): Arabidopsis Thaliana

SUBMITTER: Loïc Raijou

PROVIDER: PXD051625 | iProX | Fri Apr 19 00:00:00 BST 2024

REPOSITORIES: iProX

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A mass spectrometry-based peptidomic dataset of the spermosphere in common bean (Phaseolus vulgaris L.) seeds.

Saccaram Chandrodhay C Brosse Céline C Collet Boris B Sourdeval Delphine D François Tracy T Bernay Benoît B Corso Massimiliano M Rajjou Loïc L

Scientific data 20241107 1

The spermosphere, a dynamic microenvironment surrounding germinating seeds, is shaped by the complex interactions between natural compounds exuded by seeds and seed-associated microbial communities. While peptides exuded by plants are known to influence microbiota diversity, little is known about those specifically exuded by seeds. In this study, we characterised the peptidome profile of the spermosphere for the first time using seeds from eight genotypes of common bean (Phaseolus vulgaris) grow ...[more]

PMID: 39511243

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Project description:Seeds are crucial for plant reproduction, dispersal, and agriculture. Seed quality and vigour greatly impact crop production, referring to their ability to germinate rapidly and uniformly under varying environmental conditions, producing healthy seedlings that can withstand biotic and abiotic stress accentuated by global climate change. During germination, seeds release exudates, complex mixtures of organic and inorganic molecules, into the micro-environment surrounding them, known as the spermosphere. These exudates play a pivotal role in seedling development and overall plant fitness by influencing microbial selection, growth, and interactions in the spermosphere, ultimately shaping the plant's microbiome. To understand the composition and functional properties of germinating seed exudates, we conducted our study using different common bean (Phaseolus vulgaris L.) genotypes. Seeds were harvested in the year 2020 from eight different common bean genotypes (French commercial cultivars of Phaseolus vulgaris) cultivated in two different locations from the FNAMS field experimental stations: Gers in South-west of France (43.956991, 0.392127) and Maine et Loire (47.470532, -0.394526) in the Loire Valley. The common bean genotypes namely, VEZ, CON, CAP, FAC, FLA, LIN, DEE and VAN were chosen to represent the highest diversity among breeding programmes and qualitative seed traits in France by the seed company Vilmorin-Mikado SAS (Limagrain group). One seed lot corresponds to seeds from one genotype produced in one location. Independent biological replicates were conducted for each seed lot. Thus, 48 samples were analyzed, corresponding to three replicates of eight genotypes produced in two locations. Spermosphere collection of germinating seeds involved the imbibition of 30 seeds for each sample at four distinct time intervals: 2, 8, 16, and 24 hours. The collected spermosphere, consisting of the water surrounding germinating seeds, was gathered for each time interval and subsequently pooled. Subsequently we investigated the diversity of metabolites of each pooled spermosphere using untargetted metabolomic (LC-MS/MS) analysis.

Project description:Germination offers advantages to improve legume protein digestibility as it disintegrates seed structure and hydrolyzes proteins and anti-nutrients. Seed permeability (related to polyphenol content of seed coats) is an important factor affecting the duration of seed germination and its impact in protein digestibility and bioactivity. The objective was to compare the effect of seed germination on protease activity, structure and proteolysis of four selected legumes with contrasting seed coat polyphenol profiles (gray zero tannin [GZL], beluga [BL] and dehulled red [DL] lentils; and zero tannin/low vicine-convicine fava bean [ZF]). Protein hydrolysis was characterized during germination and digestion with respect to proteins, peptides and free amino acids (FAA). In vitro antihypertensive and antioxidant activities of digests were investigated, and peptidomic characterization (HPLC-MS/MS) and identification of bioactive fragments in intestinal digests were performed. Regardless of seed type, germination increased protease activity and reduced levels of phytic acid, trypsin inhibitors and tannins (only in BL). Significant proteolysis of the 7S and 11S globulins and concomitant increase peptides and FAA was observed in all sprouted legumes. Digestion kinetics in sprouts revealed a faster generation of FAA and peptides than in dry seeds, with changes more evident for DL associated to faster imbibition, germination and sprout growth. In contrast, BL sprouts showed the lowest protein digestibility, likely due to lower protease activity levels, seed structure disintegration and higher anti-nutrient levels in comparison to GZL, DL and ZF. Moreover, digestion of sprouts resulted in a higher number of resistant peptides in DL and ZF that matched with previously reported bioactive sequences, suggesting a promising health potential of legume sprouts that was confirmed in vitro. Results suggested that the germination process improved protein digestibility and health promoting potential of lentil and fava bean proteins although these changes were more evident in DL due to its rapid imbibition, faster germination and sprout development. This study will provide important information for either plant breeders to develop legume varieties with permeable seed coats or food producers that could use dehulled seeds for efficient production of sprouts as sustainable food sources of plant proteins with improved nutritional and healthy properties.

Dataset Information

Characterization of the peptidome in the spermosphere of common bean (Phaseolus vulgaris)

Publications

A mass spectrometry-based peptidomic dataset of the spermosphere in common bean (Phaseolus vulgaris L.) seeds.

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