Proteomics

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Effect of synchronicity of amino acid supply on the synthesis of protein in C2C12 myotubes cultured in vitro


ABSTRACT: This data further revealed the effect and mechanism of non-synchronous amino acid supply on myoglobin synthesis, and provided theoretical reference for animal precise nutrition.

ORGANISM(S): Mus Musculus

SUBMITTER: Yuan Zhao  

PROVIDER: PXD051755 | iProX | Thu Apr 25 00:00:00 BST 2024

REPOSITORIES: iProX

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Effect of synchronicity of amino acid supply on the synthesis of protein in C2C12 myotubes cultured <i>in vitro</i>.

Zhang Qiyu Q   Mi Mengmeng M   E Tianjiao T   Fu Xin X   Bao Nan N   Pan Li L   Zhao Yuan Y   Qin Guixin G  

Frontiers in veterinary science 20241029


Previous studies inferred that the synthesis rate/efficiency of protein in body tissue is probably affected by synchronicity of different amino acid (AA) supply in its metabolic pool. In order to further observe the influence of synchronicity of AA supply on the synthesis of protein in cell level, a cell culture experiment <i>in vitro</i> was conducted with C2C12 myotubes. C2C12 myotubes were cultured for 24 h, meanwhile the culture medium was replaced for each 8 h. Those myotubes were subjected  ...[more]

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