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Human_cell_PRRX1 OE_FLAG AP-MS

ABSTRACT: We performed affinity purification of PRRX1 using cells expressing Flag tagged PRRX1 isoforms - PRRX1A or PRRX1B, and carried out mass spectrometry (AP-MS) analysis.

ORGANISM(S): Homo Sapiens

SUBMITTER: Yan Zhou

PROVIDER: PXD051766 | iProX | Fri Apr 26 00:00:00 BST 2024

REPOSITORIES: iProX

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Homeodomain protein PRRX1 anchors the Ku heterodimers at DNA double-strand breaks to promote nonhomologous end-joining.

Wang Yan Y Shen Fuyuan F Zhao Chen C Li Jiali J Wang Wen W Li Yamu Y Gan Jia J Zhang Haojian H Chen Xuefeng X Chen Qiang Q Wang Fangyu F Liu Ying Y Zhou Yan Y

Nucleic acids research 20250301 6

The DNA-dependent protein kinase (DNA-PK) complex plays a critical role in nonhomologous end-joining (NHEJ), a template-independent pathway for repairing DNA double-strand breaks (DSBs). The association of Ku70/80 with DSB ends facilitates the assembly of the DNA-PK holoenzyme. However, key mechanisms underlying the attachment and stabilization of DNA-PK at broken DNA ends remain unclear. Here, we identify PRRX1, a homeodomain-containing protein, as a mediator of chromatin localization and subse ...[more]

PMID: 40114375

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