Spatial-temporal protein interactome profiling via condensation enhanced crosslinking
Ontology highlight
ABSTRACT:
ORGANISM(S): Homo Sapiens Escherichia Coli Severe Acute Respiratory Syndrome Coronavirus 2
SUBMITTER: 
Peng Chen
PROVIDER: PXD052099 | iProX | Wed Sep 11 00:00:00 BST 2024
REPOSITORIES: iProX
Publications
Spatiotemporal protein interactome profiling through condensation-enhanced photocrosslinking.
Li Kexin K Xie Xiao X Gao Rui R Chen Zhaoming Z Yang Mingdong M Wen Zhihui Z Weng Yicheng Y Fan Xinyuan X Zhang Gong G Liu Lu L Zeng Xiangmei X Han Yu Y Cao Mengrui M Wang Xin X Li Jiaofeng J Yang Zhenlin Z Li Tingting T Chen Peng R PR
Nature chemistry 20241105 1
Resolving protein-protein interactions (PPIs) inside biomolecular condensates is crucial for elucidating their functions and regulation mechanisms. The transient nature of condensates and the multiple localizations of clients, however, have rendered it challenging to determine compartment-specific PPIs. Here we developed a condensation-enhanced, spatially directed, metabolic incorporation-assisted photocrosslinking strategy-termed DenseMAP-for spatiotemporally resolved dissection of the direct p ...[more]
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