Dataset Information

Proteomic analysis of the liver in hypophysectomy rats

ABSTRACT: Individuals with hypopituitarism have an increased risk of developing non-alcoholic fatty liver disease (NAFLD)/non-alcoholic steatohepatitis (NASH) due to growth hormone (GH) deficiency. A rat model of hypopituitarism was established by hypophysectomy, followed by recombinant human growth hormone (rhGH) intervention. The mechanisms underlying GH deficiency-mediated NAFLD were elucidated through the application of label-free proteomics and phosphorylation proteomics.

ORGANISM(S): Rattus Rattus

SUBMITTER: Xuqian Fang

PROVIDER: PXD052194 | iProX | Sat May 11 00:00:00 BST 2024

REPOSITORIES: iProX

ACCESS DATA

Publications

Proteomic and metabolomic analysis of GH deficiency-induced NAFLD in hypopituitarism: insights into oxidative stress.

Zhang Yuwen Y Chen Peizhan P Fang Xuqian X

Frontiers in endocrinology 20240521

<h4>Objective</h4>Individuals with hypopituitarism (HPs) have an increased risk of developing non-alcoholic fatty liver disease (NAFLD)/non-alcoholic steatohepatitis (NASH) due to growth hormone deficiency (GHD). We aimed to investigate the possible mechanisms underlying the relationship between GHD and NAFLD using proteomic and metabolomic insights.<h4>Methods</h4>Serum metabolic alternations were assessed in male HPs using untargeted metabolomics. A rat model of HP was established through hypo ...[more]

PMID: 38836220

Similar Datasets

Project description:Mardinoglu2014 - Genome-scale metabolic model (HMR version 2.0) - human hepatocytes (iHepatocytes2322) This model is described in the article: Genome-scale metabolic modelling of hepatocytes reveals serine deficiency in patients with non-alcoholic fatty liver disease. Mardinoglu A, Agren R, Kampf C, Asplund A, Uhlen M, Nielsen J. Nat Commun 2014; 5: 3083 Abstract: Several liver disorders result from perturbations in the metabolism of hepatocytes, and their underlying mechanisms can be outlined through the use of genome-scale metabolic models (GEMs). Here we reconstruct a consensus GEM for hepatocytes, which we call iHepatocytes2322, that extends previous models by including an extensive description of lipid metabolism. We build iHepatocytes2322 using Human Metabolic Reaction 2.0 database and proteomics data in Human Protein Atlas, which experimentally validates the incorporated reactions. The reconstruction process enables improved annotation of the proteomics data using the network centric view of iHepatocytes2322. We then use iHepatocytes2322 to analyse transcriptomics data obtained from patients with non-alcoholic fatty liver disease. We show that blood concentrations of chondroitin and heparan sulphates are suitable for diagnosing non-alcoholic steatohepatitis and for the staging of non-alcoholic fatty liver disease. Furthermore, we observe serine deficiency in patients with NASH and identify PSPH, SHMT1 and BCAT1 as potential therapeutic targets for the treatment of non-alcoholic steatohepatitis. This model is hosted on BioModels Database and identified by: MODEL1402200003. To cite BioModels Database, please use: BioModels Database: An enhanced, curated and annotated resource for published quantitative kinetic models. To the extent possible under law, all copyright and related or neighbouring rights to this encoded model have been dedicated to the public domain worldwide. Please refer to CC0 Public Domain Dedication for more information.

Dataset Information

Proteomic analysis of the liver in hypophysectomy rats

Publications

Proteomic and metabolomic analysis of GH deficiency-induced NAFLD in hypopituitarism: insights into oxidative stress.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets