Dataset Information

The proteomics of IGFBP6 in endothelial cells (HUVECs)

ABSTRACT: To unravel the direct mechanism of the anti-inflammatory effects of IGFBP6 in ECs, we analysed HUVECs treated with either adenovirus-Flag-IGFBP6 (Ad-IGFBP6) or control adenovirus (Ad-NC). We precipitated IGFBP6 from overexpressed ECs followed by mass spectrometry.

ORGANISM(S): Homo Sapiens

SUBMITTER: Jianping Weng

PROVIDER: PXD052204 | iProX | Mon May 13 00:00:00 BST 2024

REPOSITORIES: iProX

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Publications

Endothelial IGFBP6 suppresses vascular inflammation and atherosclerosis.

Su Meiming M Zhao Wenqi W Jiang Hui H Zhao Yaping Y Liao Zhaopeng Z Liu Zhenghong Z Xu Mengyun M Jiang Shanshan S Wu Lili L Yang Yi Y Wang Zhihua Z Zeng Zhutian Z Fang Yun Y Tang Chaojun C Miller Clint L CL Evans Paul C PC Wang Li L Banach Maciej M Jo Hanjoong H Berk Bradford C BC Offermanns Stefan S Huang Yu Y Ge Junbo J Xu Suowen S Weng Jianping J

Nature cardiovascular research 20250110 2

Beyond dyslipidemia, inflammation contributes to the development of atherosclerosis. However, intrinsic factors that counteract vascular inflammation and atherosclerosis remain scarce. Here we identify insulin-like growth factor binding protein 6 (IGFBP6) as a homeostasis-associated molecule that restrains endothelial inflammation and atherosclerosis. IGFBP6 levels are significantly reduced in human atherosclerotic arteries and patient serum. Reduction of IGFBP6 in human endothelial cells by siR ...[more]

PMID: 39794479

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Project description:The vascular endothelium constitutes the inner lining of the blood vessel and is directly involved in the control of blood fluidity, vascular tone, platelet aggregation, regulation of immunity, inflammation, and angiogenesis. Malfunction and injuries of the endothelium can cause cardiovascular diseases, which are the leading causes of death globally. The impairment of the endothelium is a hallmark in other diseases as well such as sepsis, stroke, tumor growth, insulin resistance, venous thrombosis, and chronic kidney failure. Generation of effective sources to replace injured endothelial cells (ECs) could have significant clinical impact and somatic cell sources like peripheral or cord blood cannot credibly supply enough endothelial cell progenitors for multitude of treatments. Pluripotent stem cells are a promising source for a reliable EC supply that have the potential to repair, reconstruct, replace damaged cells, and revascularize ischemic areas in order to restore tissue function and treat vascular diseases. We have developed methods to differentiate induced pluripotent stem cells (iPSCs) efficiently and robustly across multiple iPSC lines into non-tissue-specific pan vascular ECs (iECs) with high purity. These iECs present with canonical endothelial cell markers and exhibit measures of endothelial cell functionality with uptake of acetylated low-density lipoprotein (Dil-Ac-LDL) and tube formation. Using proteomic analysis, we revealed the iECs are more proteomically similar to established umbilical vein ECs (HUVECs) than to iPSCs. Post-translational modifications (PTMs) were most shared between HUVECs and iECs, and potential targets for increasing the proteomic similarity of iECs to HUVECs were identified. PTM analysis can be used in the future to glean additional insights and generate novel hypotheses. Here we demonstrate an efficient robust method to differentiate iPSCs into functional ECs, and for the first time provide a comprehensive protein expression profile of iECs, which indicates their similarities with a widely used immortalized HUVECs, allowing for further mechanistic studies of EC development, signaling and metabolism for future regenerative applications.

Dataset Information

The proteomics of IGFBP6 in endothelial cells (HUVECs)

Publications

Endothelial IGFBP6 suppresses vascular inflammation and atherosclerosis.

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