Proteomics

Dataset Information

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Mass Spectrometry analysis of potential proteins interacting with cyclin D1 mutants (T286A or T288A)

ABSTRACT: Co-IP assays and Proteomics were performed to identify potential E3 ligases interacted with both cyclin D1 T286A and cyclin D1 T288A.

ORGANISM(S): Homo Sapiens

SUBMITTER: Haojun Xiong  

PROVIDER: PXD052332 | iProX | Fri May 17 00:00:00 BST 2024

REPOSITORIES: iProX

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Publications

F-box protein FBXO32 ubiquitinates and stabilizes D-type cyclins to drive cancer progression.

Li Feng F   Yu Hongqiang H   Zhang Yujun Y   Ma Yuanhang Y   Chen Xinlei X   Zhang Jie J   Sun Liangbo L   Guo Rui R   Wu Ying Y   Zheng Ping P   Wang Xiaojun X   Bie Ping P   He Fengtian F   Zhang Leida L   Xie Chuanming C   Xiong Haojun H  

Nature communications 20250430 1

D-type cyclins (hereafter, cyclin D) are central regulators orchestrating G1/S cell cycle transition. Accordingly, aberrant expression of cyclin D is strongly correlated with proliferation-related diseases such as cancer. However, the mechanisms regulating cyclin D turnover are incompletely elucidated. Here we identify FBXO32, namely atrogin-1, as the E3 ubiquitin ligase that targets all three cyclin D for ubiquitination and stabilization. Specifically, FBXO32 catalyzes the lysine (Lys/K)27-link  ...[more]