Functional Optic Tract Rewiring via Subtype- and Target-specific Axonal Regeneration and Presynaptic Activity Enhancement

ABSTRACT: We showed that with genetic manipulation as Pten/Socs3 knockout (KO), CNTF overexpression and Lipin1 knockdown (PSCL), RGCs could regenerate and reinnervate into OPN nuclei after pre-OPN optic tract injury. We then performed single-cell RNA sequencing on OPN-projecting retinal ganglion cells and proteomic analysis of the OPN regions to investigate the dynamic transcriptomic and proteomic changes during axonal regeneration. We had total 8 groups: GFP-intact, GFP-5dpi (5 days post injury), GFP-1.5mpi (1.5 months post injury), GFP-3mpi, PSCL-intact, PSCL-5dpi, PSCL-1.5mpi, PSCL-3mpi.

ORGANISM(S): Mus Musculus

SUBMITTER: Kai Liu  

PROVIDER: PXD052573 | iProX | Sat May 25 00:00:00 GMT+01:00 2024

REPOSITORIES: iProX