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Functional Optic Tract Rewiring via Subtype- and Target-specific Axonal Regeneration and Presynaptic Activity Enhancement

ABSTRACT: We showed that with genetic manipulation as Pten/Socs3 knockout (KO), CNTF overexpression and Lipin1 knockdown (PSCL), RGCs could regenerate and reinnervate into OPN nuclei after pre-OPN optic tract injury. We then performed single-cell RNA sequencing on OPN-projecting retinal ganglion cells and proteomic analysis of the OPN regions to investigate the dynamic transcriptomic and proteomic changes during axonal regeneration. We had total 8 groups: GFP-intact, GFP-5dpi (5 days post injury), GFP-1.5mpi (1.5 months post injury), GFP-3mpi, PSCL-intact, PSCL-5dpi, PSCL-1.5mpi, PSCL-3mpi.

ORGANISM(S): Mus Musculus

SUBMITTER: Kai Liu

PROVIDER: PXD052573 | iProX | Sat May 25 00:00:00 GMT+01:00 2024

REPOSITORIES: iProX

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Functional optic tract rewiring via subtype- and target-specific axonal regeneration and presynaptic activity enhancement.

Zhang Xin X Yang Chao C Zhang Chengle C Wu Junqiang J Zhang Xiang X Gao Jiayang J Wang Xuejie X Chan Leung Ting LT Zhou Yiren Y Chen Yujun Y Tam Sindy Sing Ting SST Chen Shuhang S Ma Yuqian Y Yung Wing-Ho WH Duan Liting L Jiang Liwen L Wang Yiwen Y Liu Kai K

Nature communications 20250304 1

Mechanisms underlying functional axonal rewiring after adult mammalian central nervous system (CNS) injuries remain unclear partially due to limited models. Here we develop a mouse intracranial pre-olivary pretectal nucleus (OPN) optic tract injury model and demonstrate that Pten/Socs3 knockout and CNTF expression in retinal ganglion cells (RGCs) promotes optic tract regeneration and OPN reinnervation. Revealed by transmission electron microscopy, trans-synaptic labeling, and electrophysiology, ...[more]

PMID: 40038284

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