Proteomics

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Exploring the Anti-Cancer mechanism of Cardiac Glycosides using proteome integral solubility alteration approach

ABSTRACT: Cancer remains a significant global health challenge, necessitating the development of innovative and cost-effective treatments. Drug repurposing, particularly of cardiac glycosides (CGs), offers a promising strategy to expedite the discovery of anti-tumor drugs. This study employs proteome integral solubility alteration (PISA) to investigate the multifaceted mechanisms of action of CGs, including digoxin, digitoxin, and ouabain. Our study revealed that CGs may interact with a spectrum of proteins associated with cellular metabolism, ribosomal function, and apoptosis, suggesting a complex interplay between these pathways in cancer cells. Besides, it suggests that CGs could be repurposed to target cancer through a multifaceted approach that disrupts cellular metabolism, protein synthesis, and mitochondrial function. Further studies are warranted to confirm the functional implications of these interactions and to explore the therapeutic potential of CGs in cancer treatment.

ORGANISM(S): Homo Sapiens

SUBMITTER: Yuxi Zhao  

PROVIDER: PXD052740 | iProX | Fri May 31 00:00:00 BST 2024

REPOSITORIES: iProX

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Exploring the anticancer mechanism of cardiac glycosides using proteome integral solubility alteration approach.

Qin Wenjie W   Deng Yinhua Y   Ren Huan H   Liu Yanling Y   Liu Ling L   Liu Wenhui W   Zhao Yuxi Y   Li Chen C   Yang Zhiling Z  

Cancer medicine 20240901 18

<h4>Background and aims</h4>Cardiac glycosides (CGs), traditionally used for heart failure, have shown potential as anti-cancer agents. This study aims to explore their multifaceted mechanisms in cancer cell biology using proteome integral solubility alteration (PISA), focusing on the interaction with key proteins implicated in cellular metabolism and mitochondrial function.<h4>Methods</h4>We conducted lysate-based and intact-cell PISA assays on cancer cells treated with CGs (Digoxin, Digitoxin,  ...[more]

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