STEAP3 promotes TNBC growth through the FGFR1-mediated activation of PI3K/AKT signaling

ABSTRACT: Breast cancer cells were cultured and harvested for proteomic analysis. Cells were lysed using a buffer containing 8 M urea, and proteins were extracted by sonication. The lysate was centrifuged to remove debris, and the supernatant was collected. Protein concentration was determined using the BCA assay. Samples were then reduced with DTT, alkylated with IAA, and digested overnight with trypsin. Peptides were desalted using C18 columns before mass spectrometry analysis.

ORGANISM(S): Homo Sapiens

SUBMITTER: Lifang Yuan  

PROVIDER: PXD052980 | iProX | Tue Jun 11 00:00:00 GMT+01:00 2024

REPOSITORIES: iProX