IsoDTB-ABPP Cysteine Chemoproteomic Profiling for bigelovin

ABSTRACT: isoDTB-ABPP Cysteine Chemoproteomic Profiling was used to detect the targets of bigelovin in NLRP3 inflammasome activation. LPS and ATP induced bone marrow derived macrophages were adopted for the experiment in three biological replicates. Cell was treated with DMSO and bigelovin respectively. The cell lysate was incubated with IA-alkyne, followed by click reaction. The DMSO group was labeled by light isoDTB tags and the bigelovin group was labeled by heavy isoDTB tags.

ORGANISM(S): Mus Musculus

SUBMITTER: Chengli Yu  

PROVIDER: PXD053000 | iProX | Tue Jun 11 00:00:00 BST 2024

REPOSITORIES: iProX