Dataset Information

ZJ5 and 025 interact protein LC-MS

ABSTRACT: IP and LS-MS to identify the interact protein of ZJ5 and 025 in Saccharomyces cerevisiae

ORGANISM(S): Saccharomyces Cerevisiae

SUBMITTER: Zhiping Xie

PROVIDER: PXD053002 | iProX | Tue Jun 11 00:00:00 BST 2024

REPOSITORIES: iProX

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Publications

Asgard Arf GTPases can act as membrane-associating molecular switches with the potential to function in organelle biogenesis.

Zhu Jing J Xie Ruize R Ren Qiaoying Q Zhou Jiaming J Chen Chen C Xie Meng-Xi MX Zhou You Y Zhang Yan Y Liu Ningjing N Wang Jinchao J Zhang Zhengwei Z Liu Xipeng X Yan Wupeng W Gong Qingqiu Q Dong Liang L Zhu Jinwei J Wang Fengping F Xie Zhiping Z

Nature communications 20250317 1

Inward membrane budding, i.e., the bending of membrane towards the cytosol, is essential for forming and maintaining eukaryotic organelles. In eukaryotes, Arf GTPases initiate this inward budding. Our research shows that Asgard archaea genomes encode putative Arf proteins (AArfs). AArfs possess structural elements characteristic of their eukaryotic counterparts. When expressed in yeast and mammalian cells, some AArfs displayed GTP-dependent membrane targeting. In vitro, AArf associated with both ...[more]

PMID: 40097441

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Project description:Highly specialized cells are fundamental for proper functioning of complex organs. Variations in cell-type specific gene expression and protein composition have been linked to a variety of diseases. Although single cell technologies have emerged as valuable tools to address this cellular heterogeneity, a majority of these workflows lack sufficient in situ resolution for functional classification of cells and are associated with extremely long analysis time, especially when it comes to in situ proteomics. In addition, lack of understanding of single cell dynamics within their native environment limits our ability to explore the altered physiology in disease development. This limitation is particularly relevant in the mammalian brain, where different cell types perform unique functions and exhibit varying sensitivities to insults. The hippocampus, a brain region crucial for learning and memory, is of particular interest due to its obvious involvement in various neurological disorders. Here, we present a combination of experimental and data integration approaches for investigation of cellular heterogeneity and functional disposition within the mouse brain hippocampus using MALDI Imaging mass spectrometry (MALDI-IMS) and shotgun proteomics (LC-MS/MS) coupled with laser-capture microdissection (LCM) along with spatial transcriptomics. Within the dentate gyrus granule cells we identified two proteomically distinct cellular subpopulations that are characterized by a substantial number of discriminative proteins. These cellular clusters contribute to the overall functionality of the dentate gyrus by regulating redox homeostasis, mitochondrial organization, RNA processing, and microtubule organization. Importantly, most of the identified proteins matched their transcripts, verifying the in situ protein identification and supporting their functional analyses. By combining high-throughput spatial proteomics with transcriptomics, our approach enables reliable near-single-cell scale identification of proteins and profiling of inter-cellular heterogeneity within similar cell-types in tissues. This methodology has the potential to be applied to different biological conditions and tissues, providing a deeper understanding of cellular subpopulations in situ.

Dataset Information

ZJ5 and 025 interact protein LC-MS

Publications

Asgard Arf GTPases can act as membrane-associating molecular switches with the potential to function in organelle biogenesis.

Similar Datasets

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