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DIA-based deep proteome and phosphoproteome profiling of early cold response in tomato fruits

ABSTRACT: Low temperature storage, a widely used technique for extending shelf life of tomato (Solanum lycopersicum) fruits, also induces chilling injury and results in fruit quality loss and consumer dissatisfaction. Numerous studies have been performed to dissect the molecular mechanisms of the cold tolerance of tomato fruits, mainly with long-time cold treatments. However, the early signal events of cold response in fruits are largely unknown. In this study, we construct mass spectral libraries of proteome (corresponding to over 11,000 proteins) and phosphoproteome (corresponding to over 24,000 high-conﬁdence phosphosites) of tomato fruits, and then perform data-independent acquisition (DIA) based comprehensive proteome and phosphoproteome profiling of tomato fruits at red-ripe stage upon cold treatment of 30 minutes.

ORGANISM(S): Solanum Lycopersicum

SUBMITTER: Zhiping Deng

PROVIDER: PXD053222 | iProX | Tue Jun 18 00:00:00 BST 2024

REPOSITORIES: iProX

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Publications

Integrative Proteome and Phosphoproteome Profiling of Early Cold Response in Maize Seedlings.

Xing Jiayun J Tan Jinjuan J Feng Hanqian H Zhou Zhongjing Z Deng Min M Luo Hongbing H Deng Zhiping Z

International journal of molecular sciences 20220610 12

Cold limits the growth and yield of maize in temperate regions, but the molecular mechanism of cold adaptation remains largely unexplored in maize. To identify early molecular events during cold shock, maize seedlings were treated under 4 °C for 30 min and 2 h, and analyzed at both the proteome and phosphoproteome levels. Over 8500 proteins and 19,300 phosphopeptides were quantified. About 660 and 620 proteins were cold responsive at protein abundance or site-specific phosphorylation levels, but ...[more]

PMID: 35742945

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