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Chemical Proteomic Profiling of Lysine Crotonylation by Bioorthogonal Probes in Mammalian Cells

ABSTRACT: we report an alkynyl-functionalized bioorthogonal chemical probe, Cr-alkyne, for the detection and identification of protein lysine crotonylation in mammalian cells.

ORGANISM(S): Homo Sapiens

SUBMITTER: Yuanfei Zhou

PROVIDER: PXD053632 | iProX | Thu Jul 04 00:00:00 BST 2024

REPOSITORIES: iProX

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Chemical proteomic profiling of lysine crotonylation using minimalist bioorthogonal probes in mammalian cells.

Zhou Yuan-Fei YF Yuan Shouli S Ma Bin B Gao Jinjun J Wang Chu C

Chemical science 20250103 6

Protein lysine crotonylation has been found to be closely related to the occurrence and development of various diseases. Currently, site identification of crotonylation is mainly dependent on antibody enrichment; however, due to the cost, heterogeneity, and specificity of antibodies, it is desired to develop an alternative chemical tool to detect crotonylation. Herein, we report an alkynyl-functionalized bioorthogonal chemical probe, Cr-alkyne, for the detection and identification of protein lys ...[more]

PMID: 39817196

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