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In vivo fitness of sul gene-dependent sulfonamide-resistant Escherichia coli in the mammalian gut

ABSTRACT: Sulfonamides are traditional synthetic antimicrobial agents used in clinical and veterinary medical settings. Their long-term excessive overuse has resulted in widespread microbial resistance, limiting their application for medical interventions. Resistance to sulfonamides is primarily conferred by the alternative genes sul1, sul2, and sul3 encoding dihydropteroate synthase in bacteria. Studying the potential fitness cost of these sul genes is crucial for understanding the evolution and transmission of sulfonamide-resistant bacteria. In vitro studies have been conducted on the fitness cost of sul genes in bacteria. In this study, we provide critical insights into bacterial adaptation and transmission using an in vivo approach.

ORGANISM(S): Escherichia Coli Str. K-12 Substr. Mg1655

SUBMITTER: Jiehong Fang

PROVIDER: PXD053633 | iProX | Thu Jul 04 00:00:00 BST 2024

REPOSITORIES: iProX

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In vivo fitness of sul gene-dependent sulfonamide-resistant Escherichia coli in the mammalian gut.

Jiang Han H Dong Yuzhi Y Jiao Xue X Tang Biao B Feng Tao T Li Ping P Fang Jiehong J

mSystems 20240814 9

The widespread sulfonamide resistance genes sul1, sul2, and sul3 in food and gut bacteria have attracted considerable attention. In this study, we assessed the in vivo fitness of sul gene-dependent sulfonamide-resistant Escherichia coli, using a murine model. High fitness costs were incurred for sul1 and sul3 gene-dependent E. coli strains in vivo. A fitness advantage was found in three of the eight mice after intragastric adm ...[more]

PMID: 39140732

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