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The label-free quantitative proteomic of EDB(+)FN1 and EDB(-)FN1

ABSTRACT: IP-MS assays of EDB(+)FN1 with a Flag epitope tag and EDB(-)FN1 with a His epitope tag. an equal amount of protein solution from each IP sample, the volume was adjusted to match the consistency of the original lysate.

ORGANISM(S): Homo Sapiens

SUBMITTER: Shi Chang

PROVIDER: PXD053745 | iProX | Mon Jul 08 00:00:00 GMT+01:00 2024

REPOSITORIES: iProX

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FN1 shapes the behavior of papillary thyroid carcinoma through alternative splicing of EDB region.

Liu Mian M Chen Pei P Wei Bo B Tan Hai-Long HL Zhao Ya-Xin YX Ai Lei L Li Ning N Jiang Ying-Ke YK Lin Jing J Li Shi-Jin SJ Chang Shi S

Scientific reports 20250102 1

Papillary thyroid cancer (PTC) is often characterized by indolent behavior, small tumors with slow cell proliferation and a tendency to metastasize to cervical lymph node simultaneously, and the molecular mechanisms underlying that remain poorly understood. In this study, FN1 was the hottest gene of PTC and distinctive expression in PTC cells. FN1 deficiency severely inhibited the p53 signaling pathway, especially cyclin proteins, resulting in increased cell growth but hampered invasion. The alt ...[more]

PMID: 39747903

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