Honeycomb-like bionic scaffold promotes integrated osteochondral repair
Ontology highlight
ABSTRACT:
ORGANISM(S): Cuniculus
SUBMITTER: 
Qingqiang Yao
PROVIDER: PXD053824 | iProX | Tue Jul 09 00:00:00 BST 2024
REPOSITORIES: iProX
Similar Datasets
Project description:Osteoporotic fractures are notoriously difficult to heal due to an imbalance between osteoblasts and osteoclasts. Current treatments often have limited efficacy or adverse side effects, highlighting the need for safer, more effective solutions. Here, we developed an injectable plant-derived phosphate coordination compound-based adhesive hydrogel to restore bone homeostasis by integrating magnesium ions (Mg2+)-phytic acid (PA) nanoparticles with aminated gelatin and aldehydated starch. The hydrogel can firmly adhere to irregular bone tissue at the fracture site and achieve achieve Mg-PA degradation in response to the osteoporotic acidic microenvironment, releasing PA and Mg2+, which modulated osteoclast and osteoblast activity, respectively. Impressively, PA inhibits osteoclastogenesis by stimulating monocyte secretion of CCN1, which competitively binds RANKL to disrupt RANKL-RANK signaling. Meanwhile, Mg2+ enhances osteoblast differentiation from bone marrow stem cells. In an ovariectomized rat model, the hydrogel significantly accelerates fracture healing (84.63% improvement over the control groups in flexural strength). This study highlights the potential of PA-based coordination compounds as a novel strategy for osteoporotic fracture treatment.
2025-09-03 | GSE284509 | GEO
Project description:Myotendinous junction (MTJ) injury cannot be repaired efficiently due to the complex composition of two tissues with distinct biological structures and mechanical properties. Previous studies designed tissue engineering scaffolds with two or more materials to mimick the distinct mechanical properties of muscle and tendon, which was a difficult and time-consuming process. Herein, we developed an integrated SilMA-PLGA scaffold incorporated with Klotho protein and bone marrow mesenchymal stem cells (BMSCs) to regulate the pathological microenvironment and tissue-resident cells for the regeneration of MTJ. The photo-crosslinked porous silk-based hydrogel (SilMA) on the 3D-printed PLGA scaffold (SM-PA) enhanced the secretory activity of BMSCs, which ensured the biological functions of macrophages, tendon stem/progenitor cells (TSPCs) and myoblasts. Besides, the incorporation of Klotho protein endowed the SM-PA scaffold with anti-oxidative and immunomodulatory properties. In an MTJ injury model of rats, this composite hydrogel scaffold (Klo/BM@SM-PA) efficiently increased the survival of transplanted BMSCs in the early post-implantation period, and presented successful MTJ regeneration capacity as evidenced by histology and immunohistochemistry evaluation. The in vivo proteomics analysis provided additional verification that the Klo/BM@SM-PA could regulate the tissue microenvironment and promote tissue regeneration
2024-02-13 | PXD049378 |
Project description:To gain molecular insights into the ability of HGF-releasing scaffold to improve microenvironmental conditions, single-nucleus RNA-seq was conducted.
2023-01-27 | GSE207200 | GEO
Project description:Lakshman Kund scaffold Metagenomic assembly
| PRJNA902919 | ENA
Project description:Therapeutic biological scaffolds promote tissue repair primarily through the induction of type 2 immunity. However, systemic immunological factors, including aging, sex, and previous infections, can modulate this response. The gut microbiota is a well-established modulator of immune function across organ systems, yet its influence on type 2-mediated repair remains underexplored. Here, we establish a bidirectional relationship between the gut microbiota and biological scaffold-mediated tissue repair. Utilizing a conventionalized germ-free mouse, we demonstrate that scaffold implantation induces compositional and functional changes in the gut microbiome, particularly affecting amino acid biosynthesis. Additionally, in a model of antibiotic-induced microbiota depletion (AIMD), we show that dysbiosis disrupts key immune regulators of type 2 immunity, including reductions in eosinophils, pro-regenerative macrophages, and IL-4-producing CD4+ T cells. At 6 weeks post-scaffold implantation, we observed a significant decrease in myocytes with centrally located nuclei alongside an upregulation in pro-fibrotic gene expression with antibiotic treatment. These findings provide insights into the influence of the gut microbiota on type 2-mediated tissue repair.
2025-12-23 | GSE312555 | GEO
Project description:This study investigated early host reactions to implanted materials to predict successful tissue regeneration with implant. Three kinds of scaffold, i.e., non-coat, collagen-coated, and PMB-coated porous polystylene scaffolds were implanted subcutaneously in mice dorsal area. Those scaffolds were used as bio-incomopatible materials, appropriate materials for tissue regeneration (bio active), and inappropriate to regenration (bio-inert) scaffolds. Seven days after implantation, scaffolds were explanted and total RNA was isolated from infiltrated host cells into scaffold by laser microdissection. Gene expressions of cells in collagen- and PMB-coated scaffold were normalized using results of non coat scaffold. Genes with more than 2-fold difference between collagen and PMB were picked up and narrowed to related keywords; inflammation, angiogenesis, wound healing, and mcrophage polarization. Among those genes, interluekin (IL)-1beta which promote both inflammation and wound healing was up-regulated in collagen-coated scaffold. On the other hand, IL-10 which suppress both inflammation and wound healing was up-regulated in PMB-coated scaffold. Angiogenesis-promoting genes were up-regulated and angiogenesis suppressve genes were suppressed in collagen. Up-regulation of IL-1b and the angiogenesis-relating genes inside the porous scaffolds are the possibly important factors for controlling tissue regeneration. Three-condition experiment, host cells infiltrated in non coat (reference), collagen-coated, and PMB-coated scaffolds. Two-microarray condition experiments, collagen vs. non coat and PMB coat vs. non coat. Hybridization: 2 replicates. Scanning: 3 replicates. Biological experiments: once.
2014-01-23 | E-GEOD-52053 | biostudies-arrayexpress
Project description:Infectious bone defects (IBDs) pose a significant clinical challenge due to the simultaneous requirements for effective infection control and robust bone regeneration, and guided bone regeneration (GBR) using multifunctional scaffolds offers a promising therapeutic strategy. Herein, we developed a bilayer hydrogel scaffold (DSHM) based on methacrylated silk fibroin (SilMA) integrated with hydroxyapatite (HA) and minocycline hydrochloride (MH) to address these demands. By precisely optimizing the concentrations of SilMA, photoinitiator, HA, and MH, we engineered a bilayer structure featuring a porous HA-loaded layer (PSH, ~432.1 μm) to enhance mechanical strength and support osteogenic differentiation, and a dense MH-loaded layer (DSM, ~34.6 μm) to act as a barrier against fibrous tissue ingrowth and provide sustained antibacterial effects. The interface-crosslinked DSHM scaffold exhibited a well-defined heterogeneous architecture, improved mechanical properties, tunable degradation, and excellent biocompatibility. Notably, DSHM demonstrated synergistic multifunctionality, including inhibition of fibroblast infiltration, promotion of bone tissue regeneration, and effective infection control. Overall, the DSHM scaffold presents a robust approach for localized and targeted treatment of IBDs, with strong potential for clinical translation.
2025-09-19 | GSE308538 | GEO
Project description:The nuclear scaffold/matrix provides an anchor for higher order genome structure that has both structural and functional implications. Different extraction protocols, i.e., utilizing either 25 mM LIS or 2 M NaCl, isolate somewhat different protein constituents of either the nuclear scaffold or nuclear matrix respectively. We have mapped, by array CGH, the locations of attachment to each of these residual protein bodies relative to non-attached DNA along the entire length of human chromosomes 14, 15, 16, 17 and 18 in HeLa cells. LIS or 2 M NaCl solutions followed by restriction digestion with EcoR1 facilitates the separation from scaffold/matrix bound DNA from non bound DNA. Genomic CGH arrays were used to map the relative differences between attached (scaffold/matrix) and non-attached (loop) portions of HeLa DNA. The expression profile of the HeLa cells used for aCGH analysis was also determined.
2010-05-22 | E-GEOD-13774 | biostudies-arrayexpress
Project description:Two shRNAs were placed into expression vectors harboring mir30 microRNA scaffold and an optimized scaffold where the artificial restriction sights in mir30 have been removed. After infection and selection shRNA processing was assessed by small-RNA cloning. For both shRNAs, placement into the optimized scaffold resulted in a ~two-fold increase in processing (based on smallRNA levels). Purpose: Others have reported that the EcoRI site that was introduced to the mir30 scaffold results in decreased smallRNA processing and hence reduced target knockdown. We've developed an alternative scaffold (termed ultramir) where this site is removed. smallRNA cloning was used to determine if the movement of this sight resulted in an increase in shRNA processing. Method: Two shRNAs (one targeting Renilla Luciferase and one targeting Human RPA3) were cloned into the original mir30 cassette the ultramir cassette. Each of the 4 constructs were infected in duplicate at single copy into cells and the cells seltected unitil infection percentages reached >90% (the shRenilla hairpin was infected into HEK293T cells and the shRPA3 construts into the Gallus gallus cell line ERC. After selection smallRNA cloning was perfromed and the amount of smallRNAs corrresponding to the two shRNAs compared to the endogenous microRNA populatlon. Results: smallRNA levels of the two shRNAs doubled relative to the microRNA population when they were placed into the ultramir scaffold.
2014-11-24 | E-GEOD-62187 | biostudies-arrayexpress
Project description:Zinc and magnesium were the essectial activators for bioenzyme. Here, we assayed single factor assays for the effect of zinc and magnesium for Monascus purpureus.Then, we carried out transcriptome assays to uncover molecular mechanism of the enhanced fermentability of bioethanol and pigments for M. purpureus under the optimal metal ion concentration.This work would provide the fermentation process for biofuels and biochemicals.
2025-01-11 | GSE279939 | GEO