Dataset Information

Proteomic of the main proteins of hemolymph in haemaphysalis flava

ABSTRACT: The main proteins of hemolymph proteome coupled with transcriptome, has provided a feasible strategy for the promotion of protein identification and the search for clues to control of tick infestations.

ORGANISM(S): Haemaphysalis Flava

SUBMITTER: Tianyin Cheng

PROVIDER: PXD053826 | iProX | Tue Jul 09 00:00:00 BST 2024

REPOSITORIES: iProX

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What are the main proteins in the hemolymph <i>of Haemaphysalis flava</i> ticks?

Li Dan D Liu Lei L Liu Zi-Ling ZL Tian Yuan Y Gao Xin X Cheng Tian-Yin TY

Frontiers in veterinary science 20240717

<h4>Background</h4><i>Haemaphysalis flava</i> is a notorious parasite for humans and animals worldwide. The organs of <i>H. flava</i> are bathed in hemolymph, which is a freely circulating fluid. Nutrients, immune factors, and waste can be transported to any part of the body via hemolymph. The main soluble components in hemolymph are proteins. However, knowledge of the <i>H. flava</i> proteome is limited.<h4>Methods</h4>The hemolymph was collected from fully engorged <i>H. flava</i> ticks by leg ...[more]

PMID: 39086760

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Project description:BackgroundTick hemolymph bathes internal organs, acts as an exchange medium for nutrients and cellular metabolites, and offers protection against pathogens. Hemolymph is abundant in proteins. However, there has been limited integrated protein analysis in tick hemolymph thus far. Moreover, there are difficulties in differentiating tick-derived proteins from the host source. The aim of this study was to profile the tick/host protein components in the hemolymph of Haemaphysalis flava.MethodsHemolymph from adult engorged H. flava females was collected by leg amputation from the Erinaceus europaeus host. Hemolymph proteins were extracted by a filter-aided sample preparation protocol, digested by trypsin, and assayed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). MS raw data were searched against the UniProt Erinaceidae database and H. flava protein database for host- and tick-derived protein identification. Protein abundance was further quantified by intensity-based absolute quantification (iBAQ).ResultsProteins extracted from hemolymph unevenly varied in size with intense bands between 100 and 130 kDa. In total, 312 proteins were identified in the present study. Therein 40 proteins were identified to be host-derived proteins, of which 18 were high-confidence proteins. Top 10 abundant host-derived proteins included hemoglobin subunit-α and subunit-β, albumin, serotransferrin-like, ubiquitin-like, haptoglobin, α-1-antitrypsin-like protein, histone H2B, apolipoprotein A-I, and C3-β. In contrast, 169 were high-confidence tick-derived proteins. These proteins were classified into six categories based on reported functions in ticks, i.e., enzymes, enzyme inhibitors, transporters, immune-related proteins, muscle proteins, and heat shock proteins. The abundance of Vg, microplusin and α-2-macroglobulin was the highest among tick-derived proteins as indicated by iBAQ.ConclusionsNumerous tick- and host-derived proteins were identified in hemolymph. The protein profile of H. flava hemolymph revealed a sophisticated protein system in the physiological processes of anticoagulation, digestion of blood meal, and innate immunity. More investigations are needed to characterize tick-derived proteins in hemolymph.

Dataset Information

Proteomic of the main proteins of hemolymph in haemaphysalis flava

Publications

What are the main proteins in the hemolymph <i>of Haemaphysalis flava</i> ticks?

Similar Datasets

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