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Proteins interacting with SARS-CoV-2 NSP16

ABSTRACT: HEK293T were transfected with SARS-CoV-2-NSP16-HA for 36 h, then treated with MG132 or DMSO for 12 h prior to harvest. Co-IP assay were performed with HA beads (Roche, catalog no. 11867423001), the elution was analyzed by mass spectrum. Mass spectrum analysis were performed by the national center for protein science (Beijing, CHN).

ORGANISM(S): Homo Sapiens

SUBMITTER: Wenyan Zhang

PROVIDER: PXD053961 | iProX | Mon Jul 15 00:00:00 BST 2024

REPOSITORIES: iProX

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SARS-CoV-2 nsp16 is regulated by host E3 ubiquitin ligases, UBR5 and MARCHF7.

Tian Li L Zhao Zongzheng Z Gao Wenying W Liu Zirui Z Li Xiao X Zhang Wenyan W Li Zhaolong Z

eLife 20250513

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of coronavirus disease 2019 (COVID-19), remains a global public health threat with considerable economic consequences. The nonstructural protein 16 (nsp16), in complex with nsp10, facilitates the final viral mRNA capping step through its 2'-<i>O</i>-methylase activity, helping the virus to evade host immunity and prevent mRNA degradation. However, nsp16 regulation by host factors remains poorly understood. While va ...[more]

PMID: 40358464

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