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Legionella pneumophila effector LegK3 phosphorylates multiple caspases involved in apoptosis signaling

ABSTRACT: Legionella pneumophila effector Legk3 phosphorylates caspase3/7/9 under infection, transfection, or in vitro purification

ORGANISM(S): Homo Sapiens

SUBMITTER: Xiaoyun Liu

PROVIDER: PXD054088 | iProX | Sat Jul 20 00:00:00 BST 2024

REPOSITORIES: iProX

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Phosphorylation of caspases by a bacterial kinase inhibits host programmed cell death.

Ge Jinli J Wang Ying Y Li Xueyu X Lu Qian Q Yu Hangqian H Liu Hongtao H Ma Kelong K Deng Xuming X Luo Zhao-Qing ZQ Liu Xiaoyun X Qiu Jiazhang J

Nature communications 20240930 1

The intracellular bacterial pathogen Legionella pneumophila utilizes the Dot/Icm system to translocate over 330 effectors into the host cytosol. These virulence factors modify a variety of cell processes, including pathways involved in cell death and survival, to promote bacterial proliferation. Here, we show that the effector LegK3 is a eukaryotic-like Ser/Thr kinase that functions to suppress host apoptosis. Mechanistically, LegK3 directly phosphorylates multiple caspases involved in apoptosis ...[more]

PMID: 39349471

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Legionella pneumophila effector LegK3 directly phosphorylates multiple caspases involved in apoptosis signaling, including Caspase-3, Caspase-7, and Caspase-9

Project description:Legionella pneumophila effector Legk3 phosphorylates caspase3/7/9 under infection, transfection, or in vitro purification condition

2024-07-28 | PXD054358 |

DYRK1B phosphorylates FOXO1 to promote hepatic gluconeogenesis

Project description:DYRK1B phosphorylates FOXO1 to promote hepatic gluconeogenesis

| PRJNA1090398 | ENA

DYRK1B phosphorylates FOXO1 to promote hepatic gluconeogenesis

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ITRAQ of HEK293T-cell protein 48 hours after transfection of phagocytophilic anaplastic effector protein Ats-1

Project description:ITRAQ of HEK293T-cell protein 48 hours after transfection of phagocytophilic anaplastic effector protein Ats-1

2021-10-28 | PXD029412 |

Aberrant Cdk5 phosphorylates Mcm2 and promotes pituitary tumorigenesis

Project description:Aberrant Cdk5 phosphorylates Mcm2 and promotes pituitary tumorigenesis

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The effector AvrRxo1 phosphorylates NAD in planta.

Project description:Gram-negative bacterial pathogens of plants and animals employ type III secreted effectors to suppress innate immunity. Most characterized effectors work through modification of host proteins or transcriptional regulators, although a few are known to modify small molecule targets. The Xanthomonas type III secreted avirulence factor AvrRxo1 is a structural homolog of the zeta toxin family of sugar-nucleotide kinases that suppresses bacterial growth. AvrRxo1 was recently reported to phosphorylate the central metabolite and signaling molecule NAD in vitro, suggesting that the effector might enhance bacterial virulence on plants through manipulation of primary metabolic pathways. In this study, we determine that AvrRxo1 phosphorylates NAD in planta, and that its kinase catalytic sites are necessary for its toxic and resistance-triggering phenotypes. A global metabolomics approach was used to independently identify 3'-NADP as the sole detectable product of AvrRxo1 expression in yeast and bacteria, and NAD kinase activity was confirmed in vitro. 3'-NADP accumulated upon transient expression of AvrRxo1 in Nicotiana benthamiana and in rice leaves infected with avrRxo1-expressing strains of X. oryzae. Mutation of the catalytic aspartic acid residue D193 abolished AvrRxo1 kinase activity and several phenotypes of AvrRxo1, including toxicity in yeast, bacteria, and plants, suppression of the flg22-triggered ROS burst, and ability to trigger an R gene-mediated hypersensitive response. A mutation in the Walker A ATP-binding motif abolished the toxicity of AvrRxo1, but did not abolish the 3'-NADP production, virulence enhancement, ROS suppression, or HR-triggering phenotypes of AvrRxo1. These results demonstrate that a type III effector targets the central metabolite and redox carrier NAD in planta, and that this catalytic activity is required for toxicity and suppression of the ROS burst.

| S-EPMC5491322 | biostudies-literature

Aurora-A phosphorylates splicing factors and regulates alternative splicing

Project description:Aurora-A phosphorylates splicing factors and regulates alternative splicing

| PRJNA674315 | ENA

CDK11 phosphorylates SF3B1 and is essential for constitutive splicing.

Project description:CDK11 phosphorylates SF3B1 and is essential for constitutive splicing.

| PRJNA770909 | ENA

TLK1 phosphorylates human RAD54

Project description:TLK1 phosphorylates RAD54 at novel residues -T41, T59, T700

2023-10-24 | PXD037131 | Pride

Generation and purification of Th1 and Th2 effector and memory CD4+ T cells

Project description:NaÃ¯ve CD4+ T cells were isolated from spleen of AND TcR transgenic/green fluorescence protein (GFP) transgenic mice (Kaye et al., Nature 1989;341:746, Wright et al, Blood 2001;97:2278) that recognize a peptide of pigeon cytochrome C in the context of I-Ek and express CD44lo, CD62Lhi, CD45RBhi, and CD25-. After 4 days in vitro stimulation with antigen presenting cells (APC) under either Th1 or Th2 condition, naÃ¯ve cells become Th1 or Th2 effector cells expressing CD44hi, CD62L lo, CD45RBhi, and CD25+. Additional 3 days culture in the absence of APC, those effector cells become rested expressing a phenotype similar to memory cells (CD44 hi, CD62L lo, CD45RB lo and CD25-). These rested effector cells were adaptively transferred into thymectomized, lethally irradiated, and T cell depleted bone marrow reconstituted mice and memory cells were isolated after 4-12 weeks by flow sort. Generation and purification of Th1 and Th2 effector and memory CD4+ T cells of 42 samples.

2010-06-10 | E-GEOD-8352 | biostudies-arrayexpress

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