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Proteomic Analysis of the Capability to Enrich Rat Nucleus Pulposus Cell Membrane Proteins on Different Glucomannan Esters

ABSTRACT: Nucleus pulposus cells show unique phenotypes and morphology on different glucomannan esters. Various protein receptors on the membrane surface are involved in cell adhesion, morphology maintenance and phenotype regulation. In this project, the cell membrane proteins of the nucleus pulposus cells were extracted and purified and incubated with different glucomannan esters to detect differentially enriched proteins to comprehensively evaluate the specific mechanism by which glucomannan esters regulate cell function.

ORGANISM(S): Rattus Norvegicus

SUBMITTER: Chunming Wang

PROVIDER: PXD054091 | iProX | Sat Jul 20 00:00:00 GMT+01:00 2024

REPOSITORIES: iProX

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An enzyme-proof glycan glue for extracellular matrix to ameliorate intervertebral disc degeneration.

Liu Yu Y Chao Tzuwei T Li Yuwei Y Peng Peng P Ge Gaoran G Geng Dechun D Dong Lei L Wang Chunming C

Nature communications 20250416 1

Intervertebral disc degeneration (IDD) remains a global healthcare challenge. Here, by analyzing clinical samples, we discover the loss of milk fat globule-EGF factor 8 (MFG-E8) from the nucleus pulposus (NP) tissue-along with increased enzymatic breakdown of glycosaminoglycans (GAGs)-as an overlooked factor for IDD development. Repairing the degraded NP extracellular matrix (ECM) with a structurally-mimicking glycan glue to enrich MFG-E8 may counter the degeneration. Accordingly, we synthesize ...[more]

PMID: 40240357

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