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Proteomic analysis of pro-stromal corneal tissue of highly myopic males and females

ABSTRACT: In this study, we aimed to explore the sex differences in myopic cornea using corneal stromal removed by small incision lenticule extraction (SMILE) surgery. The corneal stroma of highly myopic female and highly myopic male were subjected to proteomic assays. The total number of proteins in the cornea and the proteins specifically expressed in the two groups were counted, and the differentially expressed proteins in the two groups were identified by expression fold change >2 and P value <0.05, and volcano plots were constructed, functional enrichment analysis, and molecular interaction were implemented. Finally, 10 samples from each group were analyzed. 27 down-regulated proteins and 27 up-regulated proteins in the FH group, of which 23 proteins were up-regulated by a factor of 2-10 and 4 proteins were up-regulated by a factor of greater than 10. And the increased expression of proteins associated with extracellular matrix and collagen I, III, V, and VIII in female patients, and the TGF-β1/Smad3 pathway was an important pathway obtained from functional analysis. The findings of this study may help to identify sex differences that may exist in myopic corneas，and may help to further explore the pathways of myopia development and thus provide ideas for future treatments.

ORGANISM(S): Homo Sapiens

SUBMITTER: Di Chen

PROVIDER: PXD054620 | iProX | Tue Aug 06 00:00:00 BST 2024

REPOSITORIES: iProX

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Proteomic analysis reveals key differences in pro-stromal corneal tissue between highly myopic males and females.

Cui Ge G Di Yu Y Yang Shan S Chen Youxin Y Li Ying Y Chen Di D

Frontiers in medicine 20240816

<h4>Background and purpose</h4>Nowadays, myopia has become a highly prevalent disease globally, especially in East Asia. Epidemiological studies have found that there may be sex differences in the occurrence and progression of myopia, with females having a higher incidence of myopia and higher risk of myopia progression. The purpose of this study was to explore the sex differences in myopic cornea using corneal stroma removed by small incision lenticule extraction (SMILE) surgery.<h4>Methods</h4 ...[more]

PMID: 39219796

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Project description:A series of two color gene expression profiles obtained using Agilent 44K expression microarrays was used to examine sex-dependent and growth hormone-dependent differences in gene expression in rat liver. This series is comprised of pools of RNA prepared from untreated male and female rat liver, hypophysectomized (‘Hypox’) male and female rat liver, and from livers of Hypox male rats treated with either a single injection of growth hormone and then killed 30, 60, or 90 min later, or from livers of Hypox male rats treated with two growth hormone injections spaced 3 or 4 hr apart and killed 30 min after the second injection. The pools were paired to generate the following 6 direct microarray comparisons: 1) untreated male liver vs. untreated female liver; 2) Hypox male liver vs. untreated male liver; 3) Hypox female liver vs. untreated female liver; 4) Hypox male liver vs. Hypox female liver; 5) Hypox male liver + 1 growth hormone injection vs. Hypox male liver; and 6) Hypox male liver + 2 growth hormone injections vs. Hypox male liver. A comparison of untreated male liver and untreated female liver liver gene expression profiles showed that of the genes that showed significant expression differences in at least one of the 6 data sets, 25% were sex-specific. Moreover, sex specificity was lost for 88% of the male-specific genes and 94% of the female-specific genes following hypophysectomy. 25-31% of the sex-specific genes whose expression is altered by hypophysectomy responded to short-term growth hormone treatment in hypox male liver. 18-19% of the sex-specific genes whose expression decreased following hypophysectomy were up-regulated after either one or two growth hormone injections. Finally, growth hormone suppressed 24-36% of the sex-specific genes whose expression was up-regulated following hypophysectomy, indicating that growth hormone acts via both positive and negative regulatory mechanisms to establish and maintain the sex specificity of liver gene expression. For full details, see V. Wauthier and D.J. Waxman, Molecular Endocrinology (2008)

Dataset Information

Proteomic analysis of pro-stromal corneal tissue of highly myopic males and females

Publications

Proteomic analysis reveals key differences in pro-stromal corneal tissue between highly myopic males and females.

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