Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

The MS data for anti-LZTFL1 with or without ferroptosis induction

ABSTRACT: anti-LZTFL1 pulldown protein analysed by MS. NCI-H1299 cells were treated with DMSO or erastin for 8 hours..

ORGANISM(S): Homo Sapiens

SUBMITTER: Jiayi Wang

PROVIDER: PXD054848 | iProX | Thu Sep 19 00:00:00 BST 2024

REPOSITORIES: iProX

ACCESS DATA

Json Xml

Similar Datasets

MS for anti-G6PD with or without ferroptosis induction

Project description:MS for anti-G6PD with or without ferroptosis. NCI-H1299 cells were treated with DMSO or erastin for 8 hours.

2024-09-19 | PXD054838 |

Aurora A phosphorylates PKM2 at T45.

Project description:Flag-PKM2 was expressed in H1299 cells. Then the H1299 cells were treated with DMSO or 100nM MLN8237. Then, cell lysate was incubated with anti-Flag M2-agarose. Elutes were separated with 10% SDS-PAGE. The gel was stained with Coomassie Brilliant Blue. Visualized bands were excised and subjected to LC-MS/MS analysis. Then the phosphorylation sites of PKM2 were identified.

2023-03-11 | PXD036575 | Pride

Proteome analysis of Co-IP samples from NCI-H1299 cell nuclear lysate with anti-AGO2 and anti-TNRC6A antibody.

Project description:Proteome analysis of Co-IP samples from NCI-H1299 cell nuclear lysate with anti-AGO2 and anti-TNRC6A antibody.

2021-02-06 | PXD017408 | JPOST Repository

Chemotherapy response in cholangiocarcinoma

Project description:CCLP cholangiocarcinoma cells were transfected with CTRL inhibitor or MIR1249 inhibitor for 48 hours and treated with DMSO or chemotherapy (cisplatin and gemcitabine) for 48 hours before being collected and analysed for gene expression

2022-06-06 | GSE132328 | GEO

Photo-Enhanced Synergistic Induction of Ferroptosis for Anti-Cancer Immunotherapy.

Project description:Ferroptosis as programmed cell death received considerable attention in cancer research. Recently, studies have associated ferroptosis with photodynamic therapy (PDT) because PDT promotes glutathione (GSH) deletion, glutathione peroxidase 4 (GPX4) degradation, and lipid peroxide accumulation. However, PDT-induced ferroptosis may be potentially prevented by ferroptosis suppressor protein 1 (FSP1). To address this limitation, herein, a novel strategy is developed to trigger ferroptosis by PDT and FSP1 inhibition. For enhancement of this strategy, a photoresponsive nanocomplex, self-assembled by BODIPY-modified poly(amidoamine) (BMP), is utilized to stably encapsulate the inhibitor of FSP1 (iFSP1) and chlorin e6 (Ce6). The nanosystem promotes intracellular delivery, penetration, and accumulation of ferroptosis inducers in tumors with light irradiation. The nanosystem presents high-performance triggering of ferroptosis and immunogenic cell death (ICD) in vitro and in vivo. Importantly, the nanoparticles increase tumor infiltration of CD8+ T cells and further enhance the efficacy of anti-PD-L1 immunotherapy. The study suggests the potential of photo-enhanced synergistic induction of ferroptosis by the photoresponsive nanocomplexes in cancer immunotherapy.

| S-EPMC11468986 | biostudies-literature

Fungal extract screening reveals malformin C to preferentially kill glioblastoma cells via concerted induction of proteotoxic stress and autophagic flux blockade

Project description:Azide-labelled malformin C or DMSO was incubated with protein lysate from patient derived glioblastoma stem-like cells (NCH421k), and a pulldown of the compound with Dibenzocyclooctyne (DBCO) agarose was performed in order to identify interacting proteins.

2025-05-06 | PXD046258 | Pride

Anti-cancer effects of 7-oxabicyclo[2.2.1]heptene sulfonamide (OBHSA) derivative 17e on gene expression of breast cancer T47D cells

Project description:To investigate the anti-cancer effects of PROTAC-like OBHSA derivative 17e, we exposed estrogen receptor alpha positive (ERα+) breast cancer cell line T47D to compound 17e at 10 μM for 12 hours . We then performed gene expression profiling analysis using data obtained from RNA-seq of T47D cells treated with DMSO or 17e (10 μM) in three replicates.

2024-06-18 | GSE210169 | GEO

Identification of drivers and therapeutic vulnerabilities in anti-androgen resistant prostate cancer cells

Project description:Inhibition of the androgen receptor (AR) by anti-androgens is the standard treatment for castration resistant prostate cancer (CRPC), but it inevitably leads to the development of resistance. We generated two multi anti-androgen resistant cell-line models by treatment of LNCaP cells with enzalutamide (ResA) and RD-162 (ResB). Both cell-lines have an AR independent, non-neuroendocrine phenotype. To identify the resistance mechanisms we performed RNASeq analysis. To this end, the three cell-lines were plated in medium with 5% dextran-coated charcoal (DCC) treated FCS at a density of 1 million cells/well in 6-well plates. After 24 hours of steroid starvation the cells were treated with 10 nM of the androgen dihydrotestosterone (DHT), 10 µM of the anti-androgen enzalutamide, a combination of the two, or vehicle (DMSO) for 18 hours.

2019-09-26 | GSE130534 | GEO

Induction of ferroptosis using functionalized iron-based nanoparticles for anti-cancer therapy.

Project description:Ferroptosis, a cell death pathway that is induced in response to iron, has recently attracted remarkable attention given its emerging therapeutic potential in cancer cells. The need for a promising modality to improve chemotherapy's efficacy through this pathway has been urgent in recent years, and this non-apoptotic cell death pathway accumulates reactive oxygen species (ROS) and is subsequently involved in lipid peroxidation. Here, we report cancer-targeting nanoparticles that possess highly efficient cancer-targeting ability and minimal systemic toxicity, thereby leading to ferroptosis. To overcome the limit of actual clinical application, which is the ultimate goal due to safety issues, we designed safe nanoparticles that can be applied clinically. Nanoparticles containing ferroptosis-dependent iron and FDA-approved hyaluronic acid (FHA NPs) are fabricated by controlling physicochemical properties, and the FHA NPs specifically induce ROS production and lipid peroxidation in cancer cells without affecting normal cells. The excellent in vivo anti-tumor therapeutic effect of FHA NPs was confirmed in the A549 tumor-bearing mice model, indicating that the induction of FHA NP-mediated cell death via the ferroptosis pathway could serve as a powerful platform in anticancer therapy. We believe that this newly proposed FHA NP-induced ferroptosis strategy is a promising system that offers the potential for efficient cancer treatment and provides insight into the safe design of nanomedicines for clinical applications.

| S-EPMC9641001 | biostudies-literature

Effect of vehicle or drug treatment on gene expression in p53-null and p53Y220C stable cell lines

Project description:To investigate the effect of mutant p53 Y220C acetylation in gene expression, we treated NCI-H1299 (WT) and (p53Y220C-stable) cell lines with either DMSO or MS78.

2024-09-11 | GSE229576 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data