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Mass spectrometry analysis of USP38 interactors

ABSTRACT: After ectopic expression of Flag-USP38 or an empty vector in HEK293T cells for 48 hours, cells were lysed, and the supernatant after lysis was added to magnetic beads coupled with Flag antibodies. The mixture was incubated at 4°C overnight for pull-down. Subsequently, the magnetic beads were washed three times with lysis buffer and once with PBS. The magnetic bead pellet was retained for mass spectrometry identification and analysis.

ORGANISM(S): Homo Sapiens

SUBMITTER: Qingyong Hu

PROVIDER: PXD055320 | iProX | Mon Aug 26 00:00:00 BST 2024

REPOSITORIES: iProX

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ADAR promotes USP38 auto-deubiquitylation and stabilization in an RNA editing-independent manner in esophageal squamous cell carcinoma.

Hu Qingyong Q Chen Yahui Y Zhou Qianru Q Deng Shanshan S Hou Wei W Yi Yong Y Li Chenghua C Tang Jiancai J

The Journal of biological chemistry 20240919 10

Esophageal cancer is mainly divided into esophageal adenocarcinoma and esophageal squamous cell carcinoma (ESCC). China is one of the high-incidence areas of esophageal cancer, of which about 90% are ESCC. The deubiquitinase USP38 has been reported to play significant roles in several biological processes, including inflammatory responses, antiviral infection, cell proliferation, migration, invasion, DNA damage repair, and chemotherapy resistance. However, the role and mechanisms of USP38 in ESC ...[more]

PMID: 39303916

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