Dataset Information

Proteomics and phosphoproteomics reveal immune and detoxification-related functions of cystatin vaccines against adult ticks Haemaphysalis doenitzi ovary

ABSTRACT: Proteomics and phosphoproteomics reveal immune and detoxification-related functions of cystatin vaccines against adult ticks Haemaphysalis doenitzi ovary

ORGANISM(S): Haemaphysalis Doenitzi

SUBMITTER: Xiaolong Yang

PROVIDER: PXD055423 | iProX | Wed Aug 28 00:00:00 BST 2024

REPOSITORIES: iProX

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Publications

Cystatin antibodies interfere with ovary development in Haemaphysalis doenitzi (Acari: Ixodidae).

Zhang Songbo S Gao Zhihua Z Ghonaim Ahmed H AH Xing Weijia W Zhao Weikang W Zhang Jiayi J Yang Xiaolong X

PLoS neglected tropical diseases 20250507 5

Anti-tick vaccines are gaining attention as a strategy to prevent tick infestations by activating the immune response of the host. Antibodies produced by the host inhibit tick growth and reproduction, but the molecular mechanism remains to be clarified. In this study, we investigated the effects of cystatin antibodies on the ovarian function of Haemaphysalis doenitzi. Histological analysis revealed that exposure to cystatin antibodies resulted in a significant reduction in the number of eggs pro ...[more]

PMID: 40333887

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Project description:Tick saliva contains many bioactive molecules that are involved in attachment to the host, blood feeding and transmission of pathogens. MicroRNAs (miRNAs) are a class of short non-coding RNAs with a length of 19-24 nucleotides. They act as regulators of gene expression by binding to their target mRNA at the post-transcriptional level and control a variety of cellular functions, including regulation of growth, metabolism, and development. The detection and characterizations of miRNAs from tick saliva may help explain the molecular mechanisms involved in the interaction between ticks, pathogens, and hosts. They may also contribute to the discovery of vaccines, which can control ticks and the pathogens they transmit. An RNA library was generated from the saliva of fed adult Haemaphysalis longicornis ticks, and it contained 17.4 million clean reads of 18-30 nucleotides. Overall, 319 known miRNAs and 1 novel miRNA were found. The 10 most abundantly expressed miRNAs present in tick saliva were miR-100_2, miR-315, miR-184_1, miR-100-5p_2, miR-5307, miR-184-3p_3, Let-7-5p_6, miR-71_5, miR-1-3p_6, and miR-10-5p_2. The miR-375, one of the abundantly expressed, was subjected to Quantitative Real-Time PCR analysis (qRT-PCR) in various tick developmental stages, as well as in different tissues isolated from adult ticks. The expression of miR-375 in different tick development stages was highest in unfed nymphs and lowest in the egg stage. In the tissues of adult ticks, miR-375 was most highly expressed in the salivary gland. To investigate the possible role of miR-375, Ant-375 was used to inhibit the miR-375. Treated group (Ant-375) had a reduced number of eggs (t(10)= 2.652, P=0.0242), eggs that were partially desiccated, and reduced egg hatchability (t(10)=2.272, P=0.044) compared to Ms-Ant and the non-injected control. This is the first study to investigate the miRNAs profile in tick saliva and the role of miR-375 in H. longicornis. The identification and characterization of miRNA in tick saliva may help to reveal the molecular mechanisms of interactions among ticks, pathogens, and hosts and suggest new vaccine strategies to control tick borne diseases.

Dataset Information

Proteomics and phosphoproteomics reveal immune and detoxification-related functions of cystatin vaccines against adult ticks Haemaphysalis doenitzi ovary

Publications

Cystatin antibodies interfere with ovary development in Haemaphysalis doenitzi (Acari: Ixodidae).

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