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Tomato SlLYK4 Phosphorylation LC-MS/MS

ABSTRACT: To identify the key phosphorylation residues of SlLYK4, we performed liquid chromatography-tandem mass spectrometry (LC-MS/MS)in vitro kinase assays with liquid of GST-SlLYK4 and MBP-SlLYK13CD or MBP (mock) after in vitro kinase assays. And then SlLYK4-FLAG was transiently expressed in tomato hairy roots and samples were collected post EPS or chitin treatment, followed by immunoprecipitation-mass spectrometry analysis.

ORGANISM(S): Solanum Lycopersicum

SUBMITTER: Bo Li

PROVIDER: PXD055434 | iProX | Thu Aug 29 00:00:00 BST 2024

REPOSITORIES: iProX

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Differential phosphorylation of receptor kinase SlLYK4 mediates immune responses to bacterial and fungal pathogens in tomato.

Zhu Wanting W Cao Sen S Huang Mengling M Li Pengyue P Ke Jingjing J Xu Ai A Lin Yang Y Xie Jiatao J Cheng Jiasen J Fu Yanping Y Jiang Daohong D Yu Xiao X Li Bo B

Science advances 20250530 22

Bacterial wilt caused by Ralstonia solanacearum is a devastating plant disease. Exopolysaccharide (EPS), a major virulence factor of R. solanacearum, elicits pattern-triggered immunity (PTI) in tomato, but the means by which EPS is recognized in the plant remain poorly understood. We found that tomato non-arginine-aspartate (non-RD) receptor kinase SlLYK4 mediates the perception of R. solanacearum EPS and positively regulates resistance to bacterial wilt. The RD receptor kin ...[more]

PMID: 40446045

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