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4D-DIA-based quantitative proteomic analysis reveals the involvement of TRPV2 protein in duck Tembusu virus replication

ABSTRACT: Using a quantitative proteomics approach based on 4D-DIA combined with LC-MS/MS analysis, we identified TRPV2 as a potential host resistance factor that was significantly upregulated in DF-1 cells 48 hours post-DTMUV infection. Further experiments involving siRNA-mediated knockdown of TRPV2 expression in DF-1 cells revealed that suppression of TRPV2 expression inhibited DTMUV replication within the cells. Therefore, TRPV2 emerges as a promising candidate for poultry breeding programs aimed at enhancing resistance against DTMUV infection, serving as a potential target for the development of DTMUV-resistant animals in avian breeding.

ORGANISM(S): Gallus Gallus

SUBMITTER: Ruiyi Lin

PROVIDER: PXD055452 | iProX | Sun Sep 01 00:00:00 BST 2024

REPOSITORIES: iProX

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4D-DIA-Based Quantitative Proteomic Analysis Reveals the Involvement of TRPV2 Protein in Duck Tembusu Virus Replication.

Chen Jimin J Yang Fan F Lai Lianjie L Li Huihuang H Pan Chengfu C Bao Xinguo X Lin Weimin W Lin Ruiyi R

Viruses 20241126 12

Duck Tembusu virus (DTMUV), a novel positive-sense RNA virus, has caused significant economic losses in the poultry industry of Eastern and Southeast Asia since its outbreak in 2010. Furthermore, the rapid transmission and potential zoonotic nature of DTMUV pose a threat to public health safety. In this study, a 4D-DIA quantitative proteomics approach was employed to identify differentially expressed cellular proteins in DTMUV-infected DF-1 cells, which are routinely used for virus isolation and ...[more]

PMID: 39772141

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