Dataset Information

Combined analysis of transcriptome and proteome data on differential expression of genes and proteins in skeletal muscle of mice with Sjogren's syndrome treated with Yiqi Yangyin Quyu Formula

ABSTRACT: Using Yiqi Yangyin Quyu Formula to intervene in muscle tissue of SS mice for 8 weeks, observing tissue morphology and mitochondrial status, and conducting transcriptome and proteomic sequencing analysis on skeletal muscle

ORGANISM(S): Mus Musculus

SUBMITTER: Ting Zhao

PROVIDER: PXD055770 | iProX | Wed Sep 11 00:00:00 GMT+01:00 2024

REPOSITORIES: iProX

ACCESS DATA

Similar Datasets

Project description:OBJECTIVE: To observe the clinical efficacy and safety of Yiqi Yangxue formula (YQYXF) on knee osteoarthritis (KOA), and to explore the underlying therapeutic mechanism of YQYXF through endogenous differential metabolites and their related metabolic pathways.METHODS: A total of 61 KOA patients were recruited and divided into the treatment group (YQYXF, 30 cases) and the control group (celecoxib, Cxb, 31 cases). Effects of these two drugs on joint pain, swelling, erythrocyte sedimentation rate (ESR) and c-reactive protein (CRP) were observed, and their safety and adverse reactions were investigated. In animal experiments, 63 SD rats were randomly divided into normal control (NC) group, sham operation (sham) group, model (KOA) group, Cxb group, as well as low-dose (YL), medium-dose (YM), and high-dose groups of YQYXF (YH). The KOA rat model was established using a modified Hulth method. Ultra-high-performance liquid chromatography/Q Exactive HF-X Hybrid Quadrupole-Orbitrap Mass (UHPLC-QE-MS)-based metabolomics technology was used to analyze the changes of metabolites in plasma samples of rats. Comprehensive (VIP) > 1 and t-test p < 0.05 conditions were used to screen the disease biomarkers of KOA, and the underlying mechanisms of YQYXF were explored through metabolic pathway enrichment analysis. The related markers of YQYXF were further verified by ELISA (enzyme-linked immunosorbent assay).RESULTS: YQYXF can improve joint pain, swelling, range of motion, joint function, Michel Lequesen index of severity for osteoarthritis (ISOA) score, Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) score, ESR, and CRP. No apparent adverse reactions were reported. In addition, YQYXF can improve cartilage damage in KOA rats, reverse the abnormal changes of 16 different metabolites, and exert an anti-KOA effect mainly through five metabolic pathways. The levels of reactive oxygen species (ROS) and glutathione (GSH) were significantly decreased after the treatment of YQYXF.CONCLUSION: YQYXF can significantly improve the clinical symptoms of KOA patients without obvious adverse reactions. It mainly improved KOA through modulating lipid metabolism-related biomarkers, reducing lipid peroxidation and oxidative stress.

Dataset Information

Combined analysis of transcriptome and proteome data on differential expression of genes and proteins in skeletal muscle of mice with Sjogren's syndrome treated with Yiqi Yangyin Quyu Formula

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets