Dataset Information

Integrated Transcriptomic and Proteomic Analysis to Identify Genes and Molecular Mechanisms Regulating Deer Antler Growth and Ossification

ABSTRACT: Velvet antler is an organ composed of skin, nerves, blood vessels, cartilage and other tissues. Due to its ability to regenerate annually, it is used as a model for regeneration and medical research into cartilage related diseases. The study of the growth and development mechanisms of antlers has been the focus of many scholars. According to histological studies, antlers can be divided from distal to proximal into four tissue layers related to cartilage formation: reserve mesenchyme (RM), pre-cartilage (PC), transition zone (TZ), and cartilage area (CA). This study aims to identify key genes and pathways regulating antler growth and chondrogenesis by analyzing gene and protein expression levels in the four tissue layers (RM, PC, TZ, CA) of antlers.

ORGANISM(S): Elaphurus Davidianus

SUBMITTER: Pan Zhang

PROVIDER: PXD056140 | iProX | Wed Sep 18 00:00:00 BST 2024

REPOSITORIES: iProX

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Publications

Integrated Transcriptomic and Proteomic Analyses of Antler Growth and Ossification Mechanisms.

Liu Ruijia R Zhang Pan P Bai Jiade J Zhong Zhenyu Z Shan Yunfang Y Cheng Zhibin Z Zhang Qingxun Q Guo Qingyun Q Zhang Hao H Zhang Bo B

International journal of molecular sciences 20241209 23

Antlers are the sole mammalian organs capable of continuous regeneration. This distinctive feature has evolved into various biomedical models. Research on mechanisms of antler growth, development, and ossification provides valuable insights for limb regeneration, cartilage-related diseases, and cancer mechanisms. Here, ribonucleic acid sequencing (RNA-seq) and four-dimensional data-independent acquisition (4D DIA) technologies were employed to examine gene and protein expression differences amon ...[more]

PMID: 39684926

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Project description:As the only known mammalian organ that can fully and annually regenerate, deer antler has significant advantages over lower-order animal models when investigating the control of stem cell-based organ regeneration. Antler regeneration is known to be initiated and maintained by neural crest-derived stem cells in different states of activation. Antler stem cells can therefore be used as a model system to study the proteins and pathways involved in the maintenance of a stem cell niche and their activation and differentiation during organ formation. In the current study, the MSC markers CD73, CD90 and CD105 were examined within the antler tip. Label-free quantification was performed to investigate the protein profiles of antler stem cells under different stages of activation and included: dormant pedicle periosteum (DPP), antler growth center (GC), post-active stem cells from mid-beam antler periosteum (MAP), and deer facial periosteum (FP) as a control (n = 3 per group). PEAKS and IPA software were used to analyze the proteomic data. Our research confirmed the central role of stem cell activation in the development of this mammalian organ by localizing the MSC markers within the antler growth center. Label-free quantification revealed that the greatest number of unique proteins (eighty-seven) was found in the growth center tissue. There were only 12 proteins found with expression levels that significantly differed between DPP and FP. Protein profiles of these two groups indicated that antler stem cells may use similar mechanisms to maintain dormancy within a stem cell niche. The number of significantly regulated proteins between DPP, MAP and GC was 153. Among them, the majority were upregulated in the growth center. Activation of antler stem cells was associated with a number of biological processes and signaling pathways such as Hippo and canonical Wnt signaling. This work identifies the key canonical pathways, molecular/cellular functions and upstream regulators involved in mammal organ regeneration.

Dataset Information

Integrated Transcriptomic and Proteomic Analysis to Identify Genes and Molecular Mechanisms Regulating Deer Antler Growth and Ossification

Publications

Integrated Transcriptomic and Proteomic Analyses of Antler Growth and Ossification Mechanisms.

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