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The influence of IL-1β and TNF-α on the tenogenic differentiation of human periodontal ligament stem cells via global proteomic analysis

ABSTRACT: The hPDLSCs were cultured in the tenogenic medium treated with 0.1 ng/ml IL-1β or 10 ng/ml TNF-α for 7 days. Total proteins were isolated from the collected cell samples. Subsequently, the LFQ-based proteomic analysis was conducted for the extracted proteins in each group as established procedures [59]. Differential expression analysis was performed by comparing the expression levels of the identified proteins in the hPDLSCs treated with IL-1β or TNF-α with those in the Ctrl group.

ORGANISM(S): Homo Sapiens

SUBMITTER: Jialin Chen

PROVIDER: PXD056585 | iProX | Sat Oct 05 00:00:00 BST 2024

REPOSITORIES: iProX

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Controlled TPCA-1 delivery engineers a pro-tenogenic niche to initiate tendon regeneration by targeting IKKβ/NF-κB signaling.

Chen Jialin J Sheng Renwang R Mo Qingyun Q Backman Ludvig J LJ Lu Zhiyuan Z Long Qiuzi Q Chen Zhixuan Z Cao Zhicheng Z Zhang Yanan Y Liu Chuanquan C Zheng Haotian H Qi Yu Y Cao Mumin M Rui Yunfeng Y Zhang Wei W

Bioactive materials 20241023

Tendon repair remains challenging due to its poor intrinsic healing capacity, and stem cell therapy has emerged as a promising strategy to promote tendon regeneration. Nevertheless, the inflammatory environment following acute tendon injuries disrupts stem cell differentiation, leading to unsatisfied outcomes. Our study recognized the critical role of NF-κB signaling in activating inflammation and suppressing tenogenic differentiation of stem cells after acute tendon injury via multiomics analys ...[more]

PMID: 39512422

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