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Quantitative profiling of STOichiometry by Resolvable Mass tags

ABSTRACT: Post-translational modifications (PTMs) play important roles in modulating biological functions of proteins. Stoichiometry, which quantifies the modification percentage, is a critical factor for any given PTM. In this work, we developed a chemoproteomic strategy called “STO-MS” to systematically quantify the PTM stoichiometry in complex biological samples. This strategy employs a resolvable mass tag to differentiate proteoforms with different number of modifications, and utilizes liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) techniques to measure PTM stoichiometry at the proteomic level.

ORGANISM(S): Homo Sapiens

SUBMITTER: Chu Wang

PROVIDER: PXD056632 | iProX | Tue Oct 08 00:00:00 BST 2024

REPOSITORIES: iProX

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Quantitative profiling of PTM stoichiometry by resolvable mass tags.

Chen Ying Y Quan Baiyi B Li Yuanpei Y Liu Yuan Y Qin Wei W Wang Chu C

RSC chemical biology 20221005 11

Post-translational modifications (PTMs) play important roles in modulating the biological functions of proteins. Stoichiometry, which quantifies the modification percentage, is a critical factor for any given PTM. In this work, we developed a chemoproteomic strategy called "STO-MS" to systematically quantify the PTM stoichiometry in complex biological samples. This strategy employs a resolvable mass tag to differentiate proteoforms with different numbers of modifications and utilizes liquid chro ...[more]

PMID: 36349223

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