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Chemical proteomic profiling of protein N-homocysteinylation with a thioester probe

ABSTRACT: We performed quantitative chemical proteomics to identify more than 800 N-homocysteinylated proteins as well as 304 N-homocysteinylated sites directly from HTL-treated HeLa cells.

ORGANISM(S): Homo Sapiens

SUBMITTER: Chu Wang

PROVIDER: PXD056640 | iProX | Wed Oct 09 00:00:00 BST 2024

REPOSITORIES: iProX

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Chemical proteomic profiling of protein N-homocysteinylation with a thioester probe.

Chen Nan N Liu Jinmin J Qiao Zeyu Z Liu Yuan Y Yang Yue Y Jiang Changtao C Wang Xian X Wang Chu C

Chemical science 20180216 10

Hyperhomocysteinemia (HHcy) refers to a medical condition of abnormally high level of homocysteine (Hcy) in blood (>15 μmol L-1) and has been clinically implicated with cardiovascular diseases and neurodegenerative disorders. Excessive Hcy can be converted to a reactive thioester intermediate, Hcy thiolactone (HTL), which selectively reacts with protein lysine residues ("N-homocysteinylation") and this non-enzymatic modification largely contributes to manifestations of HHcy. Ho ...[more]

PMID: 29732068

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