Dataset Information

Circ_0004662 contributes to colorectal cancer progression

ABSTRACT: The MS2 bacteriophage coat protein (MS2-CP) circRNA pull-down assay was performed to detect the RNA-binding proteins (RBPs) that bind to circ_0004662.

ORGANISM(S): Homo Sapiens

SUBMITTER: Lihua Lyu

PROVIDER: PXD056767 | iProX | Mon Oct 14 00:00:00 GMT+01:00 2024

REPOSITORIES: iProX

ACCESS DATA

Publications

circ_0004662 contributes to colorectal cancer progression by interacting with hnRNPM.

Zhang Yang Y Wang Jian J Quan Ruiliang R Lyu Lihua L

International journal of oncology 20250117 2

Circular (circ)RNAs participate in colorectal cancer (CRC) occurrence and progression. However, the role of hsa_circ_0004662 (circ_0004662) in CRC remains unknown. Reverse transcription‑quantitative PCR noted high expression of circ_0004662 in CRC compared with normal colorectal epithelial cells. circ_0004662 knockdown inhibited migration of CRC cells <i>in vitro</i> and <i>in vivo</i>; would healing and Transwell assays showed that circ_0004662 overexpression contributed to CRC migration. Nucle ...[more]

PMID: 39821691

Similar Datasets

Project description:Background: circRNAs, a recently identified new member of non-coding RNAs, have been proved to play an essential role in the development and progression of various cancers. However, the functions and mechanisms of circRNAs in colorectal liver metastasis have not been fully elucidated. Methods: We performed circRNA microarray to screen differentially expressed novel circRNAs in the pathology of colorectal liver metastasis. Quantitative real-time PCR was used to detect the circ102049 expression in colorectal cancer (CRC) samples. Then CRC cells were transfected with a circ102049 over-expression vector or siRNAs, and the effects of circ102049 on biological functions in CRC cells were accessed in vitro. Mechanistically, the bioinformatics analysis, fluorescence in situ hybridization, RIP assay, RNA pull down assay and luciferase reporter assay were conducted to confirm the relationships among circ102049, miR-761, miR-192-3p and FRAS1. Moreover, the mechanism of circ102049 how to recruit and distribute DGCR8 protein in cytoplasm was elucidated. Results: The results showed that circ102049 was highly expressed in primary CRC tumors with liver metastasis and closely correlated with the CRC patients' prognosis. Circ102049 might act as a novel metastasis-related non-coding RNA, and significantly promote the adhesion, migration and invasion abilities of CRC cells. Mechanistically, we verified that circ102049 promoted the CRC development and progression via a miR-761/miR-192-3p-FRAS1-dependent mechanism. Notably, due to the distribution of DGCR8 protein, circ102049 might also reduce the mature products of miR-761 and miR-192-3p in cytoplasm indirectly. In addition, the role of circ102049 in promoting colorectal liver metastasis was also confirmed in vivo. Conclusions: Our findings provided new clues that circ102049 might be a potential prognostic factor in CRC, and the circ102049-miR-761/miR-192-3p-FRAS1 axis could be further explored as a therapeutic target for anti-metastatic therapy in CRC patients with liver metastases. Keywords: Hsa_circ_102049, miR-761, miR-192-3p, FRAS1, Colorectal liver metastasis

Dataset Information

Circ_0004662 contributes to colorectal cancer progression

Publications

circ_0004662 contributes to colorectal cancer progression by interacting with hnRNPM.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets