Dataset Information

Proteomic analysis of hydrolase levels in the lysosomes from macrophages stimulated with or without LPS via LC_MS

ABSTRACT: Proteomic analysis of hydrolase levels in the lysosomes from macrophages stimulated with or without LPS via liquid chromatography–mass spectrometry (LC-MS), highlighting the expected LPS-induced increase in accumulation of proteases, ribonucleases (RNase) and other hydrolases.

ORGANISM(S): Mus Musculus

SUBMITTER: Wei Wei

PROVIDER: PXD056776 | iProX | Mon Oct 14 00:00:00 BST 2024

REPOSITORIES: iProX

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Publications

Lysosomal "TRAP": a neotype modality for clearance of viruses and variants.

Lyu Chengliang C He Zhanlong Z Hu Xiaoming X Wang Shuang S Qin Meng M Zhu Li L Li Yanyan Y Yang Fengmei F Jiao Zhouguang Z Zhang Xiao X Lu Guihong G Wang Erqiang E Hu Yaling Y Zhai Yu Y Wang Youchun Y Huang Weijin W Wang Dongshu D Cui Yimin Y Pang Xiaocong X Liu Xiangzheng X Kamiya Hidehiro H Ma Guanghui G Wei Wei W

Nature communications 20241123 1

The binding of viruses to host-entry factor receptors is an essential step for viral infection. Many studies have shown that macrophages can internalize viruses and degrade them in lysosomes for clearance in vivo. Inspired by these natural behaviors and using SARS-CoV-2 as a testbed, we harvest lysosomes from activated macrophages and anchor the protein-receptor ACE2 as bait, thus constructing a lysosomal "TRAP" (lysoTRAP) that selectively captures, internalizes, and eventually degrades SARS-CoV ...[more]

PMID: 39578473

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Project description:Lysosomes represent a central degradative compartment of eukaryotes, yet little is known about biogenesis and function of this organelle in parasitic protists. Whereas the mannose-6 phosphate (M6P)-dependent system is dominant for lysosomal targeting in metazoans, oligosaccharide independent sorting was reported in other eukaryotes. In this study, we investigated the phagolysosomal proteome of the human parasite Trichomonas vaginalis, protein targeting and involvement of lysosomes in hydrolase secretion. The organelles were purified using conventional Percoll and OptiPrep density gradient centrifugation, and a novel purification protocol based on phagocytosis of lactoferrin-covered magnetic nanoparticles. The analysis resulted in the lysosomal proteome of 462 proteins, which were sorted into 21 functional classes. Hydrolases represent the largest functional class and include proteases, lipases, phosphatases, glycosidases, and other hydrolases. Identification of a large set of proteins involved in vesicular trafficking (80) and turnover of actin cytoskeleton rearrangement (29) indicate high dynamics of phagolysosomal compartment. Several lysosomal proteins including the cysteine protease TvCP2 were previously shown to be secreted. Our experiment showed that secretion of TvCP2 was strongly inhibited by chloroquine that increased intralysosomal pH and thus indicated TvCP2 secretion through lysosomes rather than the classical secretory pathway. Unexpectedly, we identified in the phagolysosomal proteome divergent homologs of M6P receptor TvMPR, although T. vaginalis lacks enzymes for M6P formation. To test whether oligosaccharides are involved in lysosomal targeting, we selected the lysosome-resident cysteine protease CLCP that possesses two glycosylation sites. Mutation of any of the sites redirected CLCP to the secretory pathway. Similarly, introduction of glycosylation sites to secreted β-amylase redirected this protein to lysosomes. Thus, unlike other parasitic protists, T. vaginalis seems to utilize glycosylation as recognition marker for lysosomal hydrolases. Whether TvMPR or other possible receptors are involved in this process and what is the precise structure of the lysosomal recognition marker need to be clarified in future studies.

Dataset Information

Proteomic analysis of hydrolase levels in the lysosomes from macrophages stimulated with or without LPS via LC_MS

Publications

Lysosomal "TRAP": a neotype modality for clearance of viruses and variants.

Similar Datasets

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