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PBP inactivation and OXA treatment resulted in altered protein compositions of Staphylococcal membrane vesicles

ABSTRACT: The engineered Staphylococcus aureus strain RN4220Δagr/EDⅢ was treated with 1/4 MIC of Oxacillin (OXA), or used to construct pbp3 and pbp4 gene mutant strain. Then, the membrane vesicles (MVs) of parent strain (ΔagrMVs), OXA-treated strain (Δagr/OXAMVs), and pbp3/pbp4 mutants (Δagr/OXAMVs) were harvested by density gradient centrifugation. The protein compositions of each MV sample were quantitated by using BCA Protein Assay Kit.

ORGANISM(S): Staphylococcus Aureus Subsp. Aureus Rn4220

SUBMITTER: Xiancai Rao

PROVIDER: PXD057038 | iProX | Mon Oct 21 00:00:00 BST 2024

REPOSITORIES: iProX

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Beta-Lactam Antibiotics Promote Extracellular Vesicle Production of Staphylococcus aureus Through ROS-Mediated Lipid Metabolic Reprogramming.

Huang Xiaonan X Hu Zhen Z Shang Weilong W Chen Juan J Hu Qiwen Q Zhou Yumin Y Ding Ruolan R Yin Jing J Li Mengyang M Liu He H Dou Jianxiong J Peng Huagang H Rao Yifan Y Liu Lu L Wang Yuting Y Tan Li L Yang Yuhua Y Wu Jianghong J Xiao Chuan C Yang Yi Y Rao Xiancai X

Journal of extracellular vesicles 20250501 5

Bacterial extracellular vesicles (EVs) are natural reservoirs of biological active substances. They exhibit promising application in developing bioproducts such as vaccine, drug-delivery system and anticancer agent. However, the low yield of naturally secreted EVs during bacterial growth is a bottleneck factor that restricts EV applications. In this study, we showed that sub-minimum inhibitory concentration (MIC) of β-lactams boosted EV production in various Staphylococcus aureus strains. The ex ...[more]

PMID: 40314062

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