Dataset Information

Proteomics Profile of right ventricular remodeling at compensatory stage

ABSTRACT: Proteomics Profile of right ventricular remodeling at compensatory stage (both PAB and MCT models)

ORGANISM(S): Rattus Norvegicus

SUBMITTER: Xiaoxiao Guo

PROVIDER: PXD057041 | iProX | Tue Oct 22 00:00:00 BST 2024

REPOSITORIES: iProX

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Publications

Multi-omics analysis of two rat models reveals potential role of vesicle transport and autophagy in right ventricular remodeling.

Qin Yuhan Y Zhang Jing J Wang Aiwei A Sun Wei W Qin Xiaohan X Qi Feng F Wang Yufei Y Du Le L Liu Xiaoyan X Sun Haidan H Guo Zhengguang Z Guo Xiaoxiao X

Scientific reports 20250418 1

Right ventricular failure as a severe consequence of pulmonary arterial hypertension (PAH) is an independent risk factor for poor prognosis, although the pathogenesis of right ventricular remodeling (RVR) remains unclear. Exploring the shared molecular pathways and key molecules in the right ventricle in monocrotaline (MCT) and pulmonary artery banding (PAB) rat models may reveal critical RVR mechanisms. Untargeted proteome and metabolome analysis were performed on the right ventricular myocardi ...[more]

PMID: 40251385

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Project description:Background: Lamins A/C (encoded by the LMNA gene) can lead to dilated cardiomyopathy (DCM). Objectives: This study sought to undertake proteomic analysis of myocardial tissue to explore the postgenomic phenotype of end-stage lamin heart disease. Methods: Consecutive patients with end-stage lamin heart disease (LMNA-group, n=7) and ischaemic DCM (ICM-group, n=7) undergoing heart transplantation were enrolled. Samples were obtained from left atrium(LA), left ventricle(LV), right atrium(RA), right ventricle(RV) and interventricular septum(IVS). Liquid chromatography combined with mass-spectrometry was used for protein quantification. We compared protein concentrations in cardiac samples between LMNA and ICM groups. Proteins were considered differentially abundant if the quantitative difference was 1.5-fold and corrected p-value <0.05 at a false discovery rate of 0.01. Gene ontology(GO) enrichment analysis explored the related biological processes. Results: 4,247 proteins were identified in LMNA and ICM samples, of which 633 were differentially abundant in LA, 39 in LV, 181 in RA, 52 in RV, and 85 in IVS. Abundance of lamin A/C was reduced but lamin B (LMNB) increased in LMNA LA/RA tissue compared to ICM, but not in LV/RV. Transthyretin was more abundant in the LV/RV of LMNA compared to ICM while sarcomeric proteins such as titin and cardiac myosin heavy chain were generally reduced in RA/LA of LMNA. Protein expression profiling and GO enrichment analysis revealed sarcopenia, extracellular matrix(ECM) remodeling, deficient myocardial energetics, redox imbalances, and abnormal calcium handling in LMNA samples. Conclusion: Lamin heart disease is a biventricular and biatrial disease, characterized by sarcopenia, aberrant metabolism, and ECM remodeling. LMNB and transthyretin were unexpectedly abundant in the atria and ventricles respectively of patients with end-stage lamin heart disease potentially hinting to the possibility of compensatory responses.

Dataset Information

Proteomics Profile of right ventricular remodeling at compensatory stage

Publications

Multi-omics analysis of two rat models reveals potential role of vesicle transport and autophagy in right ventricular remodeling.

Similar Datasets

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