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Reconstitution of Pluripotency from Mouse Fibroblast through Sall4 Overexpression

ABSTRACT: We report that SALL4 alone, under an optimized reprogramming medium iCD4, is capable of reprogramming mouse fibroblasts into iPSCs. To identify SALL4-direct-interacting proteins, we performed immunoprecipitation followed by mass spectrometry (IP-MS) using MEFs overexpressing WT-SALL4 or SALL4 mutants (ΔZFC1, ΔZFC2, ΔZFC3, and ΔN12) at day 1.

ORGANISM(S): Mus Musculus

SUBMITTER: Jing Liu

PROVIDER: PXD057516 | iProX | Tue Nov 05 00:00:00 GMT 2024

REPOSITORIES: iProX

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Reconstitution of pluripotency from mouse fibroblast through Sall4 overexpression.

Xiao Lizhan L Huang Zifen Z Wu Zixuan Z Yang Yongzheng Y Zhang Zhen Z Kumar Manish M Wu Haokaifeng H Mao Huiping H Lin Lihui L Lin Runxia R Long Jingxian J Zeng Lihua L Guo Jing J Luo Rongping R Li Yi Y Zhu Ping P Liao Baojian B Wang Luqin L Liu Jing J

Nature communications 20241230 1

Somatic cells can be reprogrammed into pluripotent stem cells (iPSCs) by overexpressing defined transcription factors. Specifically, overexpression of OCT4 alone has been demonstrated to reprogram mouse fibroblasts into iPSCs. However, it remains unclear whether any other single factor can induce iPSCs formation. Here, we report that SALL4 alone, under an optimized reprogramming medium iCD4, is capable of reprogramming mouse fibroblasts into iPSCs. Mechanistically, SALL4 facilitates reprogrammin ...[more]

PMID: 39737935

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