Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Glial UDP-glycosyltransferase Ugt35b regulates longevity by maintaining lipid homeostasis in Drosophila

ABSTRACT: To validate the role of glial Ugt35b in lipid metabolism, we carried out co-immunoprecipitation experiments to identify physical interaction partners of Ugt35 in glial cells. For this, we expressed HA-tagged Ugt35b under the pan-glial driver (repo-GS) and fed the flies with RU486 for 10~14 days, followed by Orbitrap Astral mass spectrometry (MS) to identify co-immunoprecipitated proteins (IP-MS). Anti-HA beads were used for the experimental group, and anti-Flag beads were used for the control group .

ORGANISM(S): Drosophila Melanogaster

SUBMITTER: Zhixiang Xu

PROVIDER: PXD057649 | iProX | Tue Dec 03 00:00:00 GMT 2024

REPOSITORIES: iProX

ACCESS DATA

Json Xml

Similar Datasets

Identification of the BRD1 interactome and its impact on schizophrenia risk

Project description:We identified protein-protein interactions and chromatin binding sites for two isforms of BRD1 (BRD1-S and BRD1-L) using Co-IP MS/MS and ChIP-seq. BRD1 isoforms were cloned, epitope-tagged (pcDNA 6 V5-His6, Lifetechnologies) and stably expressed in HEK293T cells. Chromatin immunoprecipitations were performed using anti V5-antibody conjugated agarose beads (Sigma-Aldrich) and anti HA antibody conjugated agarose beads (Sigma-Aldrich) as controls

2015-10-29 | E-GEOD-62811 | biostudies-arrayexpress

BVES-HA IP-mass from AAV9-BVES-HA injected mouse skeletal muscle

Project description:To identify BVES-interacting proteins from mouse skeletal muscle, we performed IP-mass from AAV9-BVES-HA injected mouse skeletal muscle using the anti-HA magnetic beads.

2023-02-27 | PXD036346 | Pride

Searching for the interaction partners in yeast cytosol of newly synthesized mitochondrial signal-anchored proteins

Project description:The aim of the mass spectrometry analysis was to identify interaction partners of newly synthesized precursors of mitochondrial outer membrane proteins. To that aim, HA-tagged proteins were synthesized in vitro in extract of yeast cells and pull-down with anti-HA beads was performed.

2022-08-09 | PXD031610 | Pride

Entamoeba histolytica splicing/mRNP proteome

Project description:First, the snRNP component U1A was HA-tagged and the intron of the RabX13 gene was MS2-tagged and amoeba transformats were established with such constructs. Next, amoeba transformants were UV cross-linked and their nucler extracts were immunoprecipitated (IP) with anti-HA agarose or MS2-spharose beads. Precipitates were subjected to tandem mass spectrometry (MS/MS) analyses. MS2-IP helped to discriminate the nuclear roles (chromatin-, co-transcriptional-, splicing-related), of the proteins probed.

2014-09-11 | PXD001080 | Pride

Gene expression profiling in ninaE wild type and ninaE G69D/+ Drosophila photoreceptors

Project description:Cell type specific gene expression profiling was performed with adult Drosophila photoreceptors. Two genotypes were compared: ninaE wild type control and ninaEG69D/+ mutants that have chronic endoplasmic reticulum stress. To specifically isolate RNAs from these cell types, we drove nuclear envelope-localized EGFP::Msp300KASH expression with the photoreceptor specific Rh1-Gal4 driver. Anti-GFP beads were used to isolate the nuclei from those adult fly photoreceptor cells. Novaseq was used to perform RNA-seq.

2021-10-02 | GSE185134 | GEO

Proteome of Mpox virus A5L-expressing HEK293T cells

Project description:In this research, to discover the interactions of mpox-A5 with host proteins, HEK293T cells were utilized and transfected with pCAGGS-HA-A5L plasmid (or pCAGGS-HA plasmid), harvested at 24h, Heterologous expression of MPXV A5L in HEK293T cells was performed for Co-IP with anti-HA beads, and Immunoblotting was conducted to verify the protein expression. The Co-IP products were subjected to liquid chromatography-tandem mass spectrometry (LC-MS/MS).

2024-11-13 | PXD057872 |

Identification of ETV6 bound protein by affinity purification of ETV6-HA fusion protein in REH cells

Project description:To determine the protein partners of ETV6, we expressed ETV6 and HA-tagged ETV6 in Reh pre-B leukemic cells. Anti-HA magnetic beads were used for affinity purification of ETV6-HA (and ETV6 control) from the nuclear and cytoplasmic fraction. Following purification on-beads digest and LC-MS/MS experiments were performed at the Proteomics platform of the CHU de Quebec Research Center, Quebec, Canada.

2022-01-26 | PXD031001 | Pride

Gene expression profiling of brain cortex pericytes from control and Rbpj-knockout mice at P7 and P10

Project description:Background: Pericytes are capillary-associated mural cells especially prominent in the central nervous system where they regulate vascular permeability and blood-brain barrier integrity. Despite their relevance for neurovascular unit homeostasis and their involvement in the pathobiology of neurodegenerative diseases, signalling mechanisms responsible for functional specialization and intercellular communication remain elusive. Objectives: The main goal of this study is to understand the relevance of Rbpj (the common downstream mediator of Notch signalling, an important mediator of cell-to-cell communication) in brain pericytes and to identify molecular signatures associated to Rbpj deletion. Methods: We used mouse genetic experiments to knockout Rbpj specifically in pericytes using a mural cell-specific Cre-driver (PdgfrbCreERT2). Inclusion of the Rpl22-HA (RiboTag) construct allowed Cre-mediated HA-labeling of ribosomal proteins and consequent isolation of actively translating mRNA from pericytes. PdgfrbCreERT2 transgenic mice were used to drive recombination of Rpl22-HA (RiboTag) allele and/or Rbpj-lox conditional knockout. Tamoxifen administration from postnatal day 1 (P1) to P3 allowed efficient recombination in pericytes. Brain cortices were collected at P7 or P10 and HA-tagged polyribosomes were immunoprecipitated using anti-HA coated beads. Total RNA was extracted and sequencing libraries prepared. Results: Pericyte-specific Rbpj deletion induces severe changes in the neurovascular unit characterized by defects in vascular morphogenesis, brain hemorrhaging, altered extracellular matrix composition and strong inflammatory responses. Analysis of pericytes translatome of Rbpj-KO mice compared to controls revealed profound changes in pericyte identity, increased expression of vascular smooth muscle cell markers and increased signaling through TGFbeta, among other alterations in mural cell behavior.

2019-05-11 | GSE117083 | GEO

Order 30968 - Carla Miro Vinyals 2023-02-23, Order 35373 - Carla Miro Vinyals 2024-05-29

Project description:HeLa or HEK293 cells transiently transfected with Galt1-sCGrx1p-HA redox sensor protein. Cells were treated with 0.1 mM IAA for 20 min on ice. The samples are anti-HA magnetic beads with the sensor protein captured from whole cell lysate.

2025-02-19 | PXD060874 | Pride

LC-MS/MS of YfiD-interacting proteins in mammalian cells

Project description:To figure out the intracellular function of YfiD, HA-tagged YfiD or Mock was expressed in the HeLa cell line. Subsequently, the lysates of HeLa-YfiD or HeLa-Mock cells were immunoprecipitated with anti-HA beads followed by LC-MS/MS analysis.

2024-01-15 | PXD047656 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data