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Proteomic and Acetylation Profiling of Stemness Induced by Soft Matrix in Breast Cancer Repopulating Cells

ABSTRACT: Compared to regular tumor cells, cancer stem cells exhibit dangerous characteristics, including high proliferation, high metastatic potential, and significantly increased in vivo tumorigenicity. Although some studies have emphasized the impact of the microenvironment on cell stemness, they have largely overlooked the mechanical forces derived from the stiffness of the surrounding extracellular matrix. Our previous research demonstrated that a 90 Pa soft fibrin matrix in 3D culture can induce cells to become Cancer Repopulating Cells with high stemness. Acetylation modification significantly influences the metabolism, epigenetics, proliferation, and migration of tumor cells. In this study, we performed a comprehensive analysis of the proteome and acetyl-proteome of breast cancer cells under 2D plate and 3D matrix conditions with varying stiffness.

ORGANISM(S): Homo Sapiens

SUBMITTER: Juntao Yang

PROVIDER: PXD057820 | iProX | Sat Feb 08 00:00:00 GMT 2025

REPOSITORIES: iProX

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