Proteomics

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Proteome of Mpox virus A5L-expressing HEK293T cells

ABSTRACT: In this research, to discover the interactions of mpox-A5 with host proteins, HEK293T cells were utilized and transfected with pCAGGS-HA-A5L plasmid (or pCAGGS-HA plasmid), harvested at 24h, Heterologous expression of MPXV A5L in HEK293T cells was performed for Co-IP with anti-HA beads, and Immunoblotting was conducted to verify the protein expression. The Co-IP products were subjected to liquid chromatography-tandem mass spectrometry (LC-MS/MS).

ORGANISM(S): Homo Sapiens

SUBMITTER: Lingbao Kong  

PROVIDER: PXD057872 | iProX | Wed Nov 13 00:00:00 GMT 2024

REPOSITORIES: iProX

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Transcriptomic and Proteomic Analysis of Monkeypox Virus A5L-Expressing HEK293T Cells.

Li Mingzhi M   Xiong Jiaqi J   Zhou Hao H   Liu Jing J   Wang Chenyi C   Jia Mengle M   Wang Yihao Y   Zhang Nannan N   Chen Yanying Y   Zhong Tao T   Zhang Zhicheng Z   Li Ruiying R   Zhang Yuxin Y   Guo Yunli Y   Peng Qi Q   Kong Lingbao L  

International journal of molecular sciences 20250105 1

Monkeypox (MPOX) is a zoonotic viral disease caused by the Monkeypox virus (MPXV), which has become the most significant public health threat within the <i>Orthopoxvirus</i> genus since the eradication of the Variola virus (VARV). Despite the extensive attention MPXV has garnered, little is known about its clinical manifestations in humans. In this study, a high-throughput RNA sequencing (RNA-seq) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) approach was employed to investigate  ...[more]

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