Dataset Information

Investigating the Therapeutic Impact of Traditional Chinese Medicine on Asthma through Proteomic Sequencing of Mouse Lung Tissue

ABSTRACT: Construct asthma mouse models using OVA and CFA, intervene with Warming Yang and Nourishing Kidney Approach and Xiao Qinglong Decoction respectively, and perform proteomic sequencing on the lung tissues of mice from control group-1, model group-1 and warming yang and tonifying kidney therapy group, as well as control group-2, model group-2 and Xiao Qinglong Decoction group.

ORGANISM(S): Mus Musculus 小家鼠

SUBMITTER: Xiufang Huang

PROVIDER: PXD058120 | iProX | Fri Nov 15 00:00:00 GMT 2024

REPOSITORIES: iProX

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Publications

Integrative analysis of network pharmacology and proteomics reveal the protective effect of Xiaoqinglong Decotion on neutrophilic asthma.

Liao Gang G Yan Qian Q Zhang Miaofen M Zhang Xinxin X Yang Jing J Huang Huiting H Liu Xiaohong X Jiang Yong Y Gong Jing J Zhan Shaofeng S Li Detang D Huang Xiufang X

Journal of ethnopharmacology 20240330

<h4>Ethnopharmacological relevance</h4>Xiaoqinglong Decotion (XQLD) is a commonly used Chinese herbal formula in clinical practice, especially for allergic diseases such as asthma. However, its intrinsic mechanism for the treatment of neutrophilic asthma (NA) remains unclear.<h4>Aim of the study</h4>The aim of this study was to evaluate the efficacy and potential mechanisms of XQLD on NA using network pharmacology and in vivo experiments.<h4>Materials and methods</h4>First, the active compounds, ...[more]

PMID: 38561057

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Project description:Ethnopharmacological relevance: Gubenfangxiao decoction (GBFXD) is a traditional Chinese medicine formula derived from Yupingfensan, an ancient formula widely used to treat respiratory diseases such as repeated respiratory infection, allergic rhinitis, bronchitis, and asthma. In recent years, GBFXD has been applied to efficaciously and safely treat asthma. However, the mechanism of GBFXD is still not fully elucidated. Aim of the study: A label-free proteomic method was to employed to explore the protective mechanism of GBFXD in respiratory syncytial virus (RSV)-ovalbumin (OVA)-induced chronic persistent asthmatic mice. Materials and methods: After RSV-OVA challenge, mice were orally administered GBFXD at a dose of 36 g/kg/d accompanied with OVA nasal spray once every 3 days for 28 days. The label-free proteomics-based liquid chromatography-tandem mass spectrometry method was used to explore the differentially abundant proteins (DAPs) in the serum from model mice compared with that in control mice (M:C), and in GBFXD-treated mice compared with that in model mice (G:M). We utilized OmicsBean (http://www.omicsbean.cn), a multi-omics data analysis platform, to perform bioinformatics analysis of DAPs. Enzyme-linked immunosorbent assay and Western Blotting were performed to measure the levels of related proteins and verify the results of proteomics analysis. Results: A total of 69 significant DAPs were identified including 39 in M:C, 46 in G:M, and 16 common differential proteins. Bioinformatics analysis revealed that the DAPs of M:C were mainly involved in inflammatory response and were related to lipid metabolism. However, the DAPs of G:M mostly participated in stress response, inflammatory response, and epithelial cell proliferation. Serum levels of Apoa-1, Apoc-1, Cfd, and Lrg1, EGFR and Lrg1 in the lungs were consistent with the results of proteomic analysis. Apoa-1 and Apoc-1 are closely related to cholesterol transport, lipid metabolism balance, and airway epithelial integrity; Cfd participates in immune response, affecting the occurrence and development of inflammation; EGFR and Lrg1 are involved in epithelial cell proliferation, influencing the process of airway remodeling. Conclusions: GBFXD may affect inflammatory and immune responses of asthma by regulating cholesterol transport and complement factor activation. Furthermore, it could repair damaged airway epithelium and avoid airway remodeling to prevent and treat asthma.

Dataset Information

Investigating the Therapeutic Impact of Traditional Chinese Medicine on Asthma through Proteomic Sequencing of Mouse Lung Tissue

Publications

Integrative analysis of network pharmacology and proteomics reveal the protective effect of Xiaoqinglong Decotion on neutrophilic asthma.

Similar Datasets

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